Ramírez RR, Brogden KA, Cutlip RC

Language: English/Spanish
References: 2
Page: 49-50
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Text Extraction

The Shwartzman reaction in the rabbit lung employing Pasteurella haemolytica lipopolysaccharide (LPS) resembles, at least in part, the lesions recognized in natural cases of pneumonic pasteurellosis in cattle. Recently, it has been demonstrated that the inflammatory reaction in the rabbit lung induced by the endotracheal deposition of P. haemolytica LPS (50 mg), is similar in intensity to that induced by endotracheal route followed by intravenous LPS inoculation (100 mg) to provoke a pulmonary Shwartzman reaction. It was studied in this experiment that if a greater amount of P. haemolytica LPS is administered by endotracheal route (100 mg) it still causes a major influence on the intensity of the lung inflammatory response provoked by a Shwartzman reaction. Two groups of six rabbits each, were included. Group 1 received 100 mg LPS by the endotracheal route. A similar procedure was employed for Group 2, but 24 h later they received 100 mg P. haemolytica LPS by the intravenous route. Bronchoalveolar lavages demonstrated that there was no difference in the total cell number recovered (P › 0.05). However, macrophages were higher in Group 2 than in Group 1 (P ‹ 0.05), whereas neutrophiles were higher in Group 1 (P ‹ 0.05). These results confirm that the endotracheal inoculation of LPS plays a major role on the intensity of the lung inflammatory response provoked by the Shwartzman reaction.

REFERENCES

1. Ramírez-Romero, R., Brogden, K.A. y Cutlip, R.C.: Inducción de la reacción de Shwartzman en el pulmón del conejo mediante el empleo de lipopolisacárido de Pasteurella haemolytica Vet Méx., 27: 299-307 (1996).

2. Steel, R.G.D. and Torrie, J.H.: Pnnciples and Procedures of Statistics. A Biometrical Approach. 2nd ed. McGraw-Hill Singapore, 1981.