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2008, Number 3

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Rev Mex Pediatr 2008; 75 (3)

Sensibility and specificity of smears of buffy coat stained with Gram in the diagnosis of neonatal sepsis

Ballesteros DOJC, Tena RD, García EG, Vega GA, Ramírez OMR, Sosa MJ, Tapia RC, Mendoza ZRM

Language: Spanish
References: 13
Page: 97-102
PDF size: 97.61 Kb.


ABSTRACT

Objective. To calculate the sensibility and specificity of the smears of buffy coat (BC) stained with Gram and an from another laboratory exams for the diagnosis of neonatal sepsis, taking as «gold standard» the haemocultive.
Material and methods. The validation of the results of the BC smears the tint of the BC and of those of the exams of laboratory was done in 38 neonates where the diagnosis of sepsis was you confirmed and those of 10 neonates with negative laboratory exams for the diagnosis of sepsis.
Results. Among the 38 with sepsis, the protein C reactive was high in 31 (81.5%) and the smears of the BC were positives in 29 (76.3%); neutrophilia was found in 28 (73.6%) and the vacuolization of the neutrophiles in 22 (52.6%). The biggest sensibility was for protein C reactive protein (82%) followed by the smear of BC (76%) and the haemocultive (73%): although all of them had a low specificity.
Conclusions. The smear of the BC stained with Gram is an advisable technical procedure in the diagnosis of sepsis neonatal: it is accessible, economic, simple and easy in expert hands.


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