medigraphic.com
Instituto Nacional de Salud Pública

2007, Number 6

<< Back Next >>

salud publica mex 2007; 49 (6)

SHV-type Extended-spectrum Β-lactamase (ESBL) are encoded in related plasmids from enterobacteria clinical isolates from Mexico

Garza-Ramos U, Martínez-Romero E, Silva-Sánchez J

Language: English
References: 30
Page: 415-421
PDF size: 214.55 Kb.


ABSTRACT

Objective: In this work we report the molecular characterization of Β-lactam antibiotics resistance conferred by genes contained in plasmids from enterobacteria producing extended-spectrum Β-lactamases (ESBL). Material and Methods: Fourteen enterobacterial clinical isolates selected from a group of strains obtained from seven different hospitals in Mexico during 1990-1992 and 1996-1998 were analyzed at the Bacterial Resistance Laboratory (National Institute Public Health, Cuernavaca). Molecular characterization included PFGE, IEF of Β-lactamases, bacterial conjugation, PCR amplification and DNA sequencing, plasmid extraction and restriction. Results: Isolates were genetically unrelated. ESBL identified were SHV-2 (5/14) and SHV-5 (9/14) type. Cephalosporin-resistance was transferable in 9 of 14 (64%) clinical isolates with only one conjugative plasmid, DNA finger printing showed a similar band pattern in plasmids. Conclusions: The dissemination of cephalosporin resistance was due to related plasmids carrying the ESBL genes.


REFERENCES

  1. Burwen D, Banerjee, S, Gaynes R. Ceftazidime resistance among selected nosocomial gram-negative bacilli in the United States. National Nosocomial Infections Surveillance System. J Infect Dis 1994; 170:1622- 1625.

  2. Bradford P. Extended-spectrum ß-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev 2001; 14:933-951.

  3. Bush K, Jacoby G, Medeiros A. A functional classification scheme for ß-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother 1995; 39:1211-1233.

  4. Tzouvelekis L S, Bonomo R A. SHV-type β-lactamases. Curr Pharm Des 1999;5:847-864.

  5. Howard C, van Daal A, Kelly G, Schooneveldt J, Nimmo G, Giffard P. Identification and minisequencing-based discrimination of SHV ßlactamases in nosocomial infection-associated Klebsiella pneumoniae in Brisbane, Australia. Antimicrob Agents Chemother 2002; 46:659-664.

  6. Du Bois S K, Marriott M S and Amyes S G B. TEM-and SHV-derived extended-spectrum β-lactamases: relationship between selection, structure and function. J. Antimicrob. Chemother 1995; 35:7-22.

  7. Martinez-Aguilar G, Alpuche-Aranda C, Anaya C et al. Outbreak of nosocomial sepsis and pneumonia in a newborn intensive care unit by multiresistant extended-spectrum ß-lactamase-producing Klebsiella pneumoniae: high impact on mortality. Infect Control Hosp Epidemiol 2001; 22:725-728.

  8. Alcantar-Curiel, D, Tinoco J, Gayosso C, Carlos A et al. Nosocomial bacteremia and urinary tract infections caused by extended-spectrum ß-lactamase producing Klebsiella pneumoniae with plasmids carrying both SHV-5 and TLA-1 genes. Clin Infect Dis 2004; 38:1067-1074.

  9. Miranda, G, Castro N, Leaños B et al. Clonal and Horizontal Dissemination of Klebsiella pneumoniae Expressing SHV-5 extendedspectrum ß-lactamase in a Mexican pediatric hospital. J Clin Microbiol 2004; 42:30-35.

  10. Preston K, Venezia R, Stellrecht K. The SHV-5 extended-spectrum ß-lactamase gene of pACM1 is located on the remnant of a compound transposon. Plasmid 2004; 51:48-53.

  11. Rosenblueth M, Martinez L, Silva J, Martinez-Romero E. Klebsiella variicola, a novel species with clinical and plant-associated isolates. Syst Appl Microbiol 2004; 27:27-35.

  12. Silva J, Gatica R, Aguilar C et al. Outbreak of infection with extendedspectrum β-lactamase-producing Klebsiella pneumoniae in a Mexican hospital. J Clin Microbiol 2001; 39:3193-3196.

  13. Andrade V, Silva-Sánchez J, Grupo de Resistencia Bacteriana. Caracterización de Klebsiella pneumoniae productora de la ß-lactamasa SHV-5 en una unidad de cuidados intensivos. Salud Publica Mex 2004;46:524-528.

  14. Clinical Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M100-S14. Clinical Laboratory Standards Institute, Wayne, Pa. 2005: 35.

  15. Kaufmann ME. Methods Mol Med 15, 17-31 1998.

  16. Tenover F, Arbeit R, Goering V et al. Interpreting chomosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33:2233-2239.

  17. Kieser T. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid 1984; 12:19-36.

  18. Miller J. Experiments in molecular genetics. New York, NY: Cold Spring Harbour Laboratory Press 1972:82-85.

  19. Matthew M, Harris A, Marshall M, Ross G. The use of analytical isoelectric focusing for detection and identification of ß-lactamases. J Gen Microbiol 1975; 88:169-178.

  20. Silva J, Aguilar C. ß-lactamase Bioassay: A simplified method to determine extended-spectrum ß-lactamases (ESBL) in enterobacteria. Arch Med Res 1997; 28:285-287.

  21. Arlet G, Philippon A. Construction by polymerase chain reaction and use of intragenic DNA probes for three main types of transferable ßlactamases (TEM, SHV, CARB). FEMS Microbiol Lett 1991; 66:19-25.

  22. Prodinger, W, Fille M, Bauernfeind A et al. Molecular epidemiology of Klebsiella pneumoniae producing SHV-5 β-lactamase: parallel outbreaks due to multiple plasmid transfer. J Clin Microbiol 1996; 34:564-68.

  23. Barthelemy M, Peduzzi J, Labia R. Complete amino acid sequence of p453-plasmid-mediated PIT-2 β-lactamase (SHV-1). Biochem J 1988; 251:73-79.

  24. Bradford P. Automated thermal cycling is superior to traditional methods for nucleotide sequencing of blaSHV genes. Antimicrob Agents Chemother 1999; 43:2960-2963.

  25. Billot-Klein D, Gutmann L, Collatz E. Nucleotide sequence of the SHV- 5 ß-lactamase gene of a Klebsiella pneumoniae plasmid. Antimicrob Agents Chemother 1990; 34:2439-2441.

  26. Essack S, Hall L, Pillay D, McFadyen M, Livermore D. Complexity and diversity of Klebsiella pneumoniae strains with extended-spectrum ß-lactamases isolated in 1994 and 1996 at a teaching hospital in Durban, South Africa. Antimicrob Agents Chemother 2001; 45:88-95.

  27. Chaves J, Ladona M, Segura, Coira C, Reig R, Ampurdanés C. SHV-1 ß-lactamase is mainly achromosomally encoded species-specific enzyme in Klebsiella pneumoniae. Antimicrob. Agents Chemother 2001; 45:2856-2861.

  28. Gniadkowski M, Paucha A, Grzesiowski P, Hryniewicz W. Outbreak of ceftazidime-resistant Klebsiella pneumoniae in a pediatric hospital in Warsaw, Poland: Clonal Spread of the TEM-47 Extended-Spectrum betalactamase (ESBL)-producing strain and transfer of a plasmid carrying the SHV-5-like ESBL-encoding gene. Antimicrob Agents Chemother 1998; 42:3079-3085.

  29. Sorensen S, Bailey M, Hansen L et al. Studying plasmid horizontal transfer in situ: a critical review. Nat Rev Micro 2005; 3:700-710.

  30. Ford P, Avison M. Evolutionary mapping of the SHV β-lactamase and evidence for two separate IS26-dependent blaSHV mobilization events from the Klebsiella pneumoniae chromosome. J Antimicrob Chemother 2004; 54:69-75.




2020     |     www.medigraphic.com