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Órgano Oficial de la Asociación Dental Mexicana

2008, Number 4

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Rev ADM 2008; 65 (4)

Evaluation of prostaglandin E2 on the bone resorption induction by murine osteoblasts cultivated with lipopolysaccharide

García-López S, González OBD, Wing AH, Villanueva AR

Language: Spanish
References: 27
Page: 200-205
PDF size: 140.71 Kb.


ABSTRACT

Objective: LPS is a stimulator of bone resorption in inflammatory diseases such as periodontitis, osteomyelitis and some types of arthritis. The aim of this study was to examine the effects of 1 µg/mL of LPS in murine osteoblasts under organ culture condition to evaluate weather PGE2 induce RANKL production. Material and methods: Osteoblasts were obtained from mouse skulls from Balb/c mice and plated at cell density of 104 in 1.0 cm dishe. 1 µg/mL of LPS was added to each experimental well (n = 5). Following sampling the culture supernatants at 1, 8, 24, 48, and 72 hours respectively, the supernatants were stored and assayed for PGE2 and RANKL by enzyme-linked immunosorbent assays (ELISAs). Results: Results have shown up-regulation of PGE2 and RANKL from 24 to 48 compared to the control. These data suggest that 1 µg/mL of LPS in murine osteoblasts cultivated induce RANKL production by different signalling mechanisms including PGE2 which exert their influence during bone resorption.


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