2007, Number 4
Aplicación de buenas prácticas de manufactura y determinación de agentes contaminantes en hamburguesas expendidas en salta (Argentina)
Cravero AP, Ramón AN, Bocanera B, Giménez MB, Ruiz ACG
Full text
Language: Spanish
References: 22
Page: 6-7
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ABSTRACT
The objective was to determine contaminating agents (pathogenic microorganisms and lipidic oxidation ) in hamburgers sold at local restaurants in Salta Capital city, Argentine Republic. Place of elaboration, hygienic conditions of facilities, equipment and staff in 25 authorized establishments were observed (at random). 25 samples of hamburgers (bread, beef, egg, tomato, lettuce and seasonings) were taken in sterile bags. Total recount of Aerobic Mesófilos, Coliformes, Enterobacterias, Staphylococcus aureus, Escherichia coli (O157: H7) and Index of Peroxides (IP) and Malonilaldehyd (MDA) was carried out.Non parametric tests (Sperman RHO) were statistically used; SPSS 7. 0.programs. 60% of theNon parametric tests (Sperman RHO) were statistically used; SPSS 7. 0.programs. 60% of the places had facilities, equipment and restrooms in good conditions in accordance with present regulations (80/96 MERCOSUR Regulation ). 56 to 68% met minimum conditions of hygiene in every section of the factory: 52% lacked sanitation program or did not disinfect the place in accordance .with present regulations.. 40% of the workers did .not. wear complete nor clean uniforms; 56% .lacked a. sanitary notebook and 80% had never been trained in the basicas of GMP. Undesirable habits were observed in 88%, regular work behaviors (40%) and crossed contamination (90%). Positive Total Recount in 95% of the samples (103 to 107 UFC/g); 60% with Coliforms (102 to 8 x 105 UFC/g), 10% with Enterobacterias (103 to 6 x 103 UFC/g) and 35% with Sthaphilococcus aureus (3 x 102 to 104 UFC/g) exceeding the allowed limits for consumption. E. coli negative (100%). 20% with IP at 10 (10,44 at 14,53); 92% with content of lower MDA at 0,5 (0,154 at 0,451). Highly significant correlation between microbiologic tests and sanitary notebook, training courses, behaviors and crossed contamination. The application of GPM was insufficient, reflected in the sanitary-hygienic quality of the products analyzed, since a high recount indicates contaminated raw materials , non satisfactory treatment and inadequate conditions of storage. Peroxides and MDA exceeded the allowed limits, potentially hazardous the consumer.REFERENCES