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Federación Mexicana de Ginecología y Obstetricia, A.C.

2008, Number 06

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Ginecol Obstet Mex 2008; 76 (06)

Oxidative stress level and placental histological changes during preeclampsia

Medrano RJC, Yahuaca MP, Presno BM, Alvarado AJL

Language: Spanish
References: 21
Page: 319-326
PDF size: 372.05 Kb.


ABSTRACT

Background: Oxidative stress has been related to several conditions during pregnancy (preeclampsia, abortions and premature rupture of membranes); it causes higher sensitivity of the endothelial blood vessel constriction and aggravates the endothelium dependent vasodilatación.
Objetive: To determine the oxidative stress level and histological changes in preeclamptic women’s placenta. Patients and method: Longitudinal and comparative study. There were included 25 patients referred from second level health units (IMSS, ISSSTE and Hospital General de Zacatecas). To evaluate oxidative stress level, a sample of blood and placenta were obtained during delivery and a second sample was taken during mediate puerperium (10 days).
Results: In control group, total lipidic peroxide levels in serum were 135.6 ± 7.3 nmol of MDA/mL of serum, compared with the group of moderate hypertension, which registered 222.0 ± 35.15 nmol MDA/mL. Total lipidic peroxides in serum during puerperium for control group were 150.4 ± 30.8 and 183.3 ± 18.51 nmol MDA/mL for the group of moderate hypertension. Placental lipoperoxidation for control group was 0.40 ± 0.03 µg MDA/g, and of 0.32 ± 0.03 µg MDA/g for the group of mild hypertension. Patients of moderate hypertension group showed an increase at 34% on placental lipoperoxidation over control group. Placental histological alterations where characterized by vascular remodeling loss, deposits of proteinaceous material and macrophagic process.
Conclusion: Total lipidic peroxide levels in serum increases during preeclampsia. Histological changes refer uterus-placental ischemia that, probably, induces the oxidative stress.


REFERENCES

  1. Boveris A. La evolución del concepto de radicales libres en biología y medicina. Ars Pharm 2005;46:85-95.

  2. López P, Cubillos LA, Chinchilla MA, Acevedo IC. Endotelio, célula generadora de radicales libres. Acta Med Colomb 2001; 166-168.

  3. Martínez AO, Sánchez de Medina F. Arginina, óxido nítrico y función endotelial. Ars Pharm 2004;4:303-17.

  4. Gutiérrez MA. Estrés oxidativo en la gestación: ¿Una nueva óptica en la atención a la embarazada? Rev Cubana Obstet Ginecol 2005;31:1-9

  5. Masnatta LD, Fischer PA, Dominguez GN, Cabrera Fischer EI, y col. Marcadores de estrés oxidativo. Su valor en la prevención y detección precoz de la enfermedad cardiovascular en el Hospital de Día. Rev Fed Arg Cardiol 2003;32:177-83.

  6. Reyna VE, Prieto FM, Torres MM, Reyna VN, Mejías MJ. Metabolitos del óxido nítrico plasmático y niveles de peróxidos lipídicos en pacientes preeclámpticas antes y después del parto. Rev Obstet Ginecol Venez 2002;4:89-92.

  7. Bowen RS, Moodley J, Dutton MF, Theron AJ. Oxidative stress in pre-eclampsia. Acta Obstet Gynecol Scand 2001;80:719-25.

  8. Davidge ST, Baker PN, Roberts JM. NOS expresion is increased in endothelial cells exposed to plasma from women with preeclampsia. Am J Physiol 1995;269:H1106-12.

  9. Wang Y, Walsh S, Kay H. Placental lipid peroxides and thromboxane are increased and prostacyclin is decreased in women with pre-eclampsia. Am J Obstet Gynecol 1992;147:946-9.

  10. Wu JJ. Lipid peroxidation in pre-eclamptic and eclamptic pregnancies. Eur J Obstet Gynecol Reprod Biol 1996;64:51-54.

  11. Arun KC, Undurti ND. Lipid peroxides, anti-oxidants and nitric oxide in patients with pre-eclampsia and essential hypertension. Med Sci Monit 2000;50:901-7.

  12. Martínez L, Lozano C. Hipertrigliceridemia y preeclampsia: papel fisiopatológico y evidencia actual. Med UNAB 2005;8:118-23.

  13. Guerin P, Mouatassim SE, Ménezo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surrounddings. Hum Reprod 2001;7:175-89.

  14. Many A, Hubel CA, Fisher SJ, Roberts JM, Zhou Y. Invasive cytotrophoblasts manifest evidence of oxidative stress in preeclampsia. Am J Pathol 2000;156:321-31.

  15. Yagi K. Simple assay for the level of total lipid peroxides in serum of plasma. Methods Mol Biol 1998;108:101-18.

  16. Uchiyama M, Mihara M. Determination of malonaldehide precursor in tissues by thiobarbituric acid test. Anal Biochem 1978;86:271-8.

  17. World Health Organization, International Society of Hypertension guidelines for the management of hypertension. J Hypertens 1999;12:151-63.

  18. López JP, Casas JP, Serrano N. Preeclampsia: from epidemiological observations to molecular mechanisms. Braz J Med Biol Res 2001;34:1227-1235.

  19. McQuaid KE, Keenan AK. Endothelial barrier dysfunction and oxidative stress: roles for nitric oxide? Exp Physiol 1997;82:369-76.

  20. Lyall F, Bulmer JN, Kelly H, Duffie E, Robson SC. Human trophoblast invasion and spiral artery transformation: the role of PECAM-1 in normal pregnancy, preeclampsia, and fetal growth restriction. Am J Pathol 1999;158:1105-14.

  21. Bdolah Y, Karumanchi A, Sachs BP. Recent Advances in understanding of preeclampsia. Croat Med J 2005;728-76.




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