Groman-Lupa S, Voorduin S, Pedroza-Seres M, Valdés-Gonzalez T, López-Star E, Jiménez-Martinez MC

Language: Spanish
References: 11
Page: 385-390
PDF size: 70.88 Kb.

ABSTRACT

Objective: To evaluate CD8+CD57+ T cell frequency in uveitis patients, the expression of cell migration markers on T cells, and to correlate the immunological data with the ocular findings.
Methods: Uveitis patients were included. The expression of CD8, CD57, CD103, CD108, CCR9, CLA were assessed by flow cytometry on peripheral blood mononuclear cells. Data were analyzed with Sigma-Stat software. The correlations were performed using Spearman or Pearson correlations. Differences were considered statistically when p < 0.05.
Results: CD8+CD57+ T cell frequency was increased in uveitis patients. Expression of CD103 (p=0.02), CD108 (p=0.03), CCR9 (p<0.001) and CLA (p=0.01) on T cells was statistically significant in panuveitis patients than in intermediate uveitis patients. Patients with more clinical punctuation showed significant correlation with the expression of CLA (r=- 1, p=0.0001) and CD8 (r=-1, p=0.008). A positive correlation was observed in intermediate uveitis patients, when CD108 expression was associated with CCR9 expression (r= 0.9, p=0.03) on T cells.
Conclusions: CD8+CD57+ T cell frequency was increased in uveitis patients. T cells from intermediate uveitis patients were mainly CD108+CCR9+, suggesting eye homing. T cells from panuveitis patients were mainly CD103+CD108+CCR9+CLA+, suggesting both, eye and skin homing. Clinical-immunological correlation was significant only in panuvetis patients. CLA/CD8 could be biological markers of intraocular immunological activity.

REFERENCES

1. Rothova A, Suttorp-van Schulten MSA, Treffers WF, Kijlstra A. Causes and frequency of blindness in patients with intraocular inflammatory disease. Br J Ophthalmol 1996; 80(4):332–336.

2. Voorduin S, Zagorin F. Frecuencia y causa de la uveítis en el Instituto de Oftalmología Conde de Valenciana. Rev Mex Oftalmol 2005; 79(4): 193-196.

3. Pedroza-Seres M, Linares M, Voorduin S, Rojas-Ramos E y cols. Pars Planitis is associated with and increased frequency of effector-memory CD57+ T cells. Br J Ophthalmol 2007; 91(10):1393-8.

4. Jiménez-Martínez MC, Carrera-Cardenas I, Pedroza-Seres M y cols. Uveitis Immunopathology: current knowledge, clinical correlation and research perspectives in the immune-ocular area. Rev Alerg Mex 2006; 53(6):226-35.

5. The Standardization of Uveitis Nomenclature (SUN) Working Group. Standarization of Uveitis Nomenclature for Reporting Clinical Data. Results of the First International Workshop. J Ophthalmol 2005; 140:509-516.

6. Sada-Ovalle I, Torre-Bouscoulet L, Valdez-Vázquez R y cols. Characterization of a cytotoxic CD57+ T cell subset from patients with pulmonary tuberculosis. Clin Immunol 2006; 121(3):314-23.

7. Abbas A, Baker D. Cellular and molecular immunology. 6a ed. EUA: Saunders-Elsevier, 2007.

8. El-Asady R, Yuan R, Liu K, Wang D, Gress RE y cols. TGF- {beta}-dependent CD103 expression by CD8(+) T cells promotes selective destruction of the host intestinal epithelium during graft-versus-host disease. J Exp Med 2005; 201(10):1647-57.

9. Suzuki K, Okuno T, Yamamoto M, Pasterkamp RJ y cols. Semaphorin 7A initiates T-cell-mediated inflammatory responses through alpha1beta1 integrin. Nature 2007; 446(7136):680-4.

10. de Haas AH, Boddeke HW, Biber K. Region-specific expression of immunoregulatory proteins on microglia in the healthy CNS. Glia 2008; 56(8):888-94.

11. Clark RA, Chong B, Mirchandani N, Brinster NK y cols. The vast majority of CLA+ T cells are resident in normal skin. J Immunol 2006; 176(7):4431-9.