2007, Number 3
La suplementación con vitaminas antioxidantes a mujeres con endometriosis disminuye la producción de citocinas proinflamatorias y aumenta la síntesis de IFN-γ en leucocitos periféricos en cultivo
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ABSTRACTObjective: To evaluate the vitamins C and E supplementation on the production of cytokines inflammatory mediators and IFN-λ.
Method: 34 women with endometriosis diagnosis participated of the Clinic of Sterility at the Instituto Nacional de Perinatología, Isidro Espinosa de los Reyes (INPerIER). The study was a controlled randomized and double blind clinical trial. During six months the patients received a bar with vitamins C and E (343 mg/84 mg) or placebo. In the first and last consultation, a blood sample stimulated during 24 hours was taking with lipo-polysacarid presence. At the term, we recovered the plasm to evaluate the production of Interferon-gamma (IFN-λ), Interleukyn-1 beta (IL-1Β), IL-6 and Tumoral Necrosis Factor-alpha (TNF-α), by ELISA technique.
Results: In the intervention group the production of IL-6 and IL-1Β diminished, while the IFN-λ increased, after six months of intervention (CI 95%). The placebo group didn’t present change in the cytokines pattern at the end of the study. The comparison among groups at the end of study showed a decrease in the production of IL-6 and IL-1Β, associated to an increase of IFN-λ, on the group that received the vitamins supplementary (CI 95%). There was not statistical difference between the cytokines production from the beginning of study, for inter groups. There was not differences observed inter or intra groups for the TNF-α production.
Conclusion: After six moths, the vitamins C and E supplementation diminished the IL-1b production and IL-6 and increased the IFN-g production in the women of the study.
Hernández-Guerrero C, Vadillo-Ortega F, Beltrán-Montoya J, Farina-Granja M, Ávila- Vergara MA, Bustos-López H, Arriaga- Pizarro L. Inducción de la síntesis de óxido nítrico en células mononucleares en cultivo utilizando líquido peritoneal de mujeres con endometriosis, en relación al porcentaje de linfocitos T y células NK identificando en dicho ambiente. Ginecol Obstret Mex 2001; 69: 12-18.