1997, Number 4
Establishment and evaluation of avidin-biotin sistem onto nitrocellulose paper for the serodiagnosis of porcine cysticercosis
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ABSTRACTAn immunodot test was implemented to detect pigs with antibodies against Cysticercus cellulosae. The antigen was prepared with Cysticercus cellulosae and diluted in tris buffer saline (TBS). Then it was spotted in volumes of 10 µl onto the nitrocellulose paper which then was blocked, fixed and incubated at room temperature. Pig's serum was diluted in TBS 7.5 pH containing gelatin and tween 20; then it was added followed by biotin-avidin peroxidase labeled antibody directed toward the pig's IgG. The addition of a substrate resulted in the formation of a purple color when the test was positive. The immunodot test showed a concordance of 95.4%, a sensitivity of 91,1% and a specificity of 96.9% when the results where compared with the results of the postmortem sanitary inspection. The immunodot test described in this report is simple, cheap and provides objetive results for the serodiagnosis of porcine cysticercosis.
Ruiz EJ. Detección de anticuerpos contra Cysticercus cellulosae mediante la utilización del ensayo inmunoenzimático (ELISA) en sueros de la población humana de la Facultad de Medicina Veterinaria y Zootecnia de la UAT (tesis de licenciatura). Ciudad Victoria (Tamaulipas) México: Univ Aut Tamaulipas, 1988.