2008, Number 3
Rev Biomed 2008; 19 (3)
Antimicrobial susceptibility and virulence factors of Vibrio cholerae no-O1 isolates isolated from patients with acute diarrhea
Cabrera-Rodríguez LE, Bravo-Fariñas L, Ramírez-Álvarez MM, Llop-Hernández A, Fernández-Abreu A, Morier L, Borrego-Hernández G
PDF size: 204.00 Kb.
ABSTRACTIntroduction. The intestinal infections caused for Vibrio cholerae no-O1 are increasing in the world.
Objective. To know the antimicrobial susceptibility to the drug of choice and alternative ones and to determine the presence of some virulence factors as: extracellular enzymes (Dnase, gelatinase, lecitinase, elastase and hemolysin), HEp-2 cells adherence and presence of fimbrias.
Materials and Methods. A total of 65 isolates of Vibrio cholerae no-O1 were obtained from patients with acute diarrhea in 7 provincial laboratories of microbiology in Cuba from January to December 2006.
Results. The figures for resistance of sulphonamide and ampicillin were 32.3% and 30.7%, respectively. Sensitivity levels over 85% were recorded for the antibiotics fluoroquinolones, tetracycline, chloramphenicol and trimetoprimsulphamethoxazole. Hemolysin and gelatinase enzymes were found in 100% of the isolates.
Conclusions. The studied isolates show low resistance towards the tested antimicrobials except for sulfonamide and ampicilline. At least two of the investigated virulence factors were found in all isolates.