2007, Number 3
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ABSTRACTIntroduction. Adenoviruses are responsible for at least 5% of acute respiratory infections world wide. Regularly, they produce self-limited middle respiratory tract infections. Depending on the type of infecting adenovirus and the immunological status of the patient, adenoviral infection may result in high morbidity of respiratory diseases.
Objective. To identify the adenovirus type from isolates of patients with acute respiratory infection.
Material and Methods. In order to isolate the adenovirus agent, samples of respiratory secretion were inoculated into HEP-2 cells. Detection of adenoviral isolation was done by indirect immunofluorescence. Adenoviral typing was done by restriction enzyme analysis using the enzymes Eco-RI, Hind-III, Bgl-II and Sma-I (RFLP).
Results. A total of 118 samples were analysed, of which 22% were identified as adenovirus positive by specific immunofluorescence. The RFLP pattern allowed the typing of Ad1 in 4 samples, Ad2 in 6 samples and 17 samples had the type Ad5.
Conclusions. We are reporting for the first time the presence of Ad1, Ad2, and Ad5 in patients with acute respiratory infection.
De Jong JC, Wermenbol AG, Verweij-Uijterwaal MW, Slaterus KW, Wertheim-Van Dillen P, Van Doornum GJ, et al. Adenoviruses from human immunodeficiency virus-Infected Individuals, including two strains that represent new candidate serotypes Ad50 and Ad51 of species B1 and D, respectively. J Clin Microbiol 1999; 37:3940-5.