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>Journals >Gaceta Médica de México >Year 2008, Issue 6

Bastarrache RA, López-Alvarenga JC, Kent Jr JW, Laviada-Molina HA, Cerda-Flores RM, Calderón-Garcidueñas AL, Torres-Salazar A, Gallegos-Cabrales EC, Tejero ME, Cole SA , Comuzzie AG
Transcriptoma en mexicanos. Metodología para analizar el perfil de expresión genética de gran escala en muestras simultáneas de tejido muscular, adiposo y linfocitos obtenidas en un mismo individuo
Gac Med Mex 2008; 144 (6)

Language: Español
References: 22
Page: 473-480
PDF: 177.38 Kb.

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Objective: We describe the methodology used to analyze multiple transcripts using microarray techniques in simultaneous biopsies of muscle, adipose tissue and lymphocytes obtained from the same individual as part of the standard protocol of the Genetics of Mexican Metabolic Disorders (GMMD) Family Study. Methods: We recruited 4 healthy male subjects with BMI 20-41, who signed an informed consent letter. Subjects participated in a clinical examination that included anthropometric and body composition measurements, muscle biopsies (vasus lateralis) subcutaneous fat, and a blood draw. All samples provided sufficient amplified RNA for microarray analysis. Total RNA was extracted from the biopsy samples and amplified for analysis. Results: Of the 48 687 transcript targets queried, 39.4% were detectable in a least one of the studied tissues. Leptin was not detectable in lymphocytes, weakly expressed in muscle, but overexpressed and highly correlated with BMI in subcutaneous fat. Another example was GLUT4, which was detectable only in muscle and not correlated with BMI. Expression level concordance was 0.7 (p‹0.001) for the three tissues studied. Conclusions: We demonstrated the feasibility of carrying out simultaneous analysis of gene expression in multiple tissues, concordance of genetic expression in different tissues, and confidence that this method corroborates the expected biological relationships among LEP and GLUT4. The GEMM study will provide a broad and valuable overview on metabolic diseases, including obesity and type 2 diabetes.

Key words: Microarray analysis, messenger RNA, metabolic syndrome (syndrome X), genetic expression, untranslated RNA.


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>Journals >Gaceta Médica de México >Year 2008, Issue 6

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