>Year 2004, Issue 3
Tzoc E, Arias ML, Valiente C
Effects of hospitalary waste water residues on the antibiotic resistance patterns of Escherichia coli and Aeromonas sp.
Rev Biomed 2004; 15 (3)
PDF: 39.59 Kb.
Introduction. Health attention centers, especially hospitals, represent important sources of antibiotic discharges into the environment, producing a big effect on the physical, chemical, and biological composition of receptor bodies.
Material and methods. In order to assess the impact of a hospital effluent on the antibiotic resistance of freshwater bacterial populations, water samples were collected, upstream and downstream, from a creek bordering a Class A hospital, in San José, Costa Rica. 120 samples of Escherichia coli and 75 Aeromonas sp strains were evaluated and tested for antibiotic susceptibility by the Kirby-Bauer methodology.
Results. Most E. coli strains were resistant to ampicillin and amoxicillin, with a 57 and 45% resistance, respectively, they also showed significant resistance to tetracycline.
In the Aeromonas sp isolates, the greatest resistance rates were for the same antibiotics as E. coli, probably due to the intrinsic resistance of these bacteria to ß lactamics.
Discussion. The hospital effluent shows an important effect on the occurrence of resistant strains, although it is not the only source, since the resistance patterns of the bacteria isolated from the creek before receiving the hospitalary effluent already showed high levels.
||Antibiotics, antimicrobial resistance, Escherichia coli, Aeromonas sp., waste water.
Bush K. Antimicrobial agents. Curr Op Chem Biol 1997; 1: 169-175.
Allsop A. New antibiotic discovery, novel screens, novel targets, and impact in microbial genomics. Curr Op Microbiol 1998; 1: 530-4.
Moir D, Shaw K, Hare R, Vovis G. Genomics and antimicrobial drug discovery. Antimicrob Agents Chemother 1999; 43: 4398-446.
Morris A, Kellner J, Low D. The superbugs: evolution, dissemination and fitness. Curr Op Microbiol 1998; 1: 524-9.
Harrison J, Svec T. The beginning of the end of the antibiotic era? Part 1. The problem: abuse of the “miracle drugs”. Quintessence Int 1998; 29: 151-62
Neu H. The crisis of antibiotic resistance. Science 1992; 257: 1064-73.
Roberts M. Tetracycline resistance determinants: mechanism of action, regulation of expression, genetic mobility, and distribution. FEMS Microbial Rev 1996; 19: 1-24.
Andersson D, Levin B. The biological cost of antibiotic resistance. Curr Op Microbiol 1999; 2: 489-93.
Al-Ghazzi M, Jazrawi S, Al-Doori. Antibiotics resistance among pollution indicator bacteria isolated from Al-Khair river, Baghdad. Wat Res 1988; 2: 641-4.
Al Jebouri M. A note on antibiotic resistance in the bacterial flora of raw sewage and sewage polluted river Tigris in Mosul, Iraq. J Appl Bacteriol 1985; 58: 401-5.
Andersen J, Sandaa R. Distribution of tetracycline resistance determinants among Gram negative bacteria isolated from polluted and unpolluted marine sediments. Appl Environ Microbiol 1994, 60: 908-12.
Hernández A. Caracterización preliminar de la descarga líquida del Hospital México. Tesis. Universidad de Costa Rica. 1999.
Hailling-Sorensen B, Nors S, Lanzky P, Ingerslev F, Holten H, Jorgensen S. Occurrence, fate, and effects of pharmaceutical substances in the environment. A review. Chemosphere 1999; 36: 357-93.
Ternes T. Occurrence of drugs in German sewage treatment plants and rivers. Water Res 1998; 32: 3245-60.
Goñi-Uriza M, Capdepuy M, Arpin C, Raymond N, Caumette P, Quentin C. Impact of an urban effluent on antibiotic resistance of riverine Enterobacteriaceae and Aeromonas spp. Appl Environ Microbiol 2000; 66: 124-32.
Guardabassi L, Petersen H, Olsen J, Dalsguard A. Antibiotic resistance in Acinetobacter spp. isolated from sewers receiving waste effluent from a hospital and a pharmaceutical plant. Appl Environ Microbiol 1998; 64: 3499-502.
Mach P, Grimes J. R-plasmid transfer in a wastewater treatment plant. Appl Environ Microbiol 1982; 44: 1395-403.
Méndez A. Evaluación del patrón de susceptibilidad a antibióticos de bacilos Gram negativos aislados a partir de una quebrada aledaña a un hospital clase A comparada con los aislados a partir de la Quebrada Los Negritos. Tesis. Universidad de Costa Rica, 2000.
Anonymous. Standard Methods for the Microbiological Examination of Water and Wastewater. APHA, Washington D.C., 2000.
Anonymous. Clinical Microbiology Procedures Handbook. ASM, Washington, 1995; p. 460-539.
Trevors J, Berkay T, Bourquin W. Gene transfer among bacteria in soil and aquatic environments. A review. Can J Microbiol 1987; 33: 191-8.
Timoney J, Port J, Giles J, Spanier J. Heavy metal and antibiotic resistance in the bacterial flora of sediments of New York Bight. Appl Environ Microbiol 1978; 36: 409-14.
Winorowski A. Resistance to antibiotics of heavy metal tolerant and heavy metal sensitive bacterial strains. J Envir Sc Hlth 1993; A28: 203-15.
Moffie B & Mouton R. Sensitivity and resistance of Legionella pneumophila to some antibiotics and combinations of antibiotics. J Antimicrob Chemother 1988; 22:457-62.
De Paola M, Peeler J, Rodnick G. Effect of oxytetracycline medicated feed on antibiotic resistance of Gram negative bacteria in catfish ponds. Appl Environ Microbiol 1995; 61: 2335-40.
Son R. Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from culture fish tilapia (Telapia mossambica). Letters in Applied Microbiol 1997; 24: 479-82.
Chant B, Bolton S. Plasmids and resistance to antimicrobial agents in Aeromonas sobria and Aeromonas hydrophila clinical isolates. Antimicrobic Agents Chemother 1987; 31: 1281-2.
Bhattacherjee J, Spathak J, Gaur A. Antibiotic resistance and metal tolerance of coliform bacteria isolated from Gomati River water at Lucknow city. J Gen Appl Microbiol 1988; 51: 1285-92.
Pathak S, Bhattachejee J, Ray P. Seasonal variation in survival and antibiotic resistance among various bacterial populations in a tropical river. J Gen Appl Microbiol 1993; 39: 47-56.
Bell J, Elliot G, Smith D. Influence of sewage treatment and urbanization on selection multiple resistance in fecal coliform populations. Appl Environ Microbiol 1983, 46: 227-32.
Cohen ML, Tauxe R. Drug resistant Salmonella in the United States: an epidemiological perspective. Science 1986; 234: 964-9.
McKeon D, Calabrese J, Bissonnettes G. Antibiotic resistant Gram negative bacteria in rural ground water supplies. Wat Res 1995; 29:1902-8.
>Year 2004, Issue 3