medigraphic.com
Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2003, Number 1

<< Back Next >>

Rev Biomed 2003; 14 (1)

Analysis of sister chromatid exchanges in mint factory workers

Tummalapalli P, Ramana DCV, Reddy PP

Language: English
References: 20
Page: 11-15
PDF size: 29.04 Kb.


ABSTRACT

Introduction. Sister chromatid exchanges (SCEs) were analysed in the peripheral lymphocytes of mint factory workers exposed to metal alloys of aluminium, copper, nickel and magnesium.
Materials and methods. Heparinised blood samples were collected from mint factory workers (63 nonsmokers and 67 smokers), 54 nonsmokers and 42 smokers belonging to the same socioeconomic group and not exposed to toxic chemicals. All the samples were studied for frequency of sister chromatid exchanges. The exposed population was subgrouped into 3 categories based on duration of exposure.
Results. There was a significant increase in the frequency of SCEs in the exposed group when compared to controls. The frequency of SCEs increased with duration of exposure to metal dusts. Further more the increase was significant at all durations of exposure when compared to control group.
Discussion. The present study reveals that prolonged exposure of man to these metals may result in genetic damage.


REFERENCES

  1. Roy AK, Sharma A, Talukdar G. Effects of aluminium salts on bone marrow chromosomes in rats invivo cytobios 1991; 66:105-11.

  2. Colomina MT, Gomez M, Domingo JL, Corbell J. Teratogenic effect of aluminium hydroxide in Swiss albino mice. Pharmacol Toxicol 1994; 74:236-9.

  3. Carta.P, Couo PL, Flora C, Pau M, Grussu M, Cherchi P. Mortality in workers of a primary aluminium foundry in Portovesme in Sardinia. Med Lav 1992; 83:530-5.

  4. Merchal-Seault D. The effect of commercial preparations of benlate, dithane M-45, copper sulphate and dichlofluanid on the fertility of Drosophila melanogaster. J Environ Sci Health 1993; 28:397-411.

  5. Panigrahi A, Darmahapatra AK, Medda AK. Effect of lead, zinc, mercury and copper with and without estrogen on serum vitellogenin level in magur fish (Clarius batrachus). Geenbaurs Morphol Jahrb 1990; 136:775-80.

  6. Tachon P. DNA single strand breakage by H2O2 and ferric or cupric ions, its modultion by histidine. Free-Radic Res Common 1990; 9:39-47.

  7. Horiguchi S, Morinaga K. Endo G. Epidemiological study of mortality from cancer among chromium platers. Asia Pac J Public Health 1990; 4:169-72.

  8. Furst A, Schlauder MC. The hamster as a model for metal carcinogenenesis. Proc West Pharmacol 1971; 16:150-3.

  9. Jaquet P, Mayence A. Application of the in vitro embryo culture to the study of the mutagenic effects of nickel in male germ cells. Toxicol Lett 1982; 11:193-7.

  10. Senft V, Losan F, Tusek M. Cytogenetic analysis of chromosomal aberattions of peripheral lymphocytes in workers occupationally exposed to nickel. Mut Res 1992; 279:171-9.

  11. Moorhead PS, Nowell PS, Mellmann WJ, Battups DM, Hungerford DA. Chromosomal preparation of leukocytes cultured from human peripheral blood. Exp Cell Res 1960; 20:613-5.

  12. Perry P, Wolff S. New giemsa method for the differential staining of sister chromatids. Nature 1974; 251:156-8.

  13. Schoket B. Monitoring occupational to exposure carcinogens. IARC Sci Publ 1993; 124:341-7.

  14. Szyba K, Golonka MC, Gaslorowski K, Urban J. Mutagenic activity of copper (II) chromate and dichromate complexes. with polypyridines. Biometals 1992; 5:157-61.

  15. Sunderman FN. Evolution of clinical science. An overview. Ann Clin Lab Sci 1993; 23:231-48.

  16. Kusiak RA, Rilchie AC, Springer J, Muller J. Mortality from stomach cancer in ontario miners. Por J Ind Med 1993; 50:117-26.

  17. Sandstrom A, Wall B. Cancer incidence among male salaried employees at a smeltery in northern Sweden. Acta Oncol 1993; 32:9-14.

  18. Arrouisalfz FZ, Marzin D, Pestel J. Differences in genotoxic activity of alpha-Ni 352 on human lymphocytes from nickelhypersensitized and nickel unsensitized donors. Mutagenesis 1992; 34:183-7.

  19. Rupa DS, Reddy PP, Reddy OS. Chromosomal aberrations in peripheral lymphocytes of cotton field workers exposed to pesticides. Environ Res 1989;49:1-6.

  20. Padmaja-Reddy T, Ramana-Devi ChV, Vidyullata V, Hema-Prasad M, Reddy PP. Chromosomal aberrations in smokers exposed to metallic dust in mint factory. Rev Biomed 2000; 11:87-90.




2020     |     www.medigraphic.com