Pérez-Lorenzo R, Chan-Avilés MI, Sánchez-Sosa D, Abasolo-Flores GP, López-Pacheco E

Language: English
References: 27
Page: 69-74
PDF size: 36.23 Kb.

ABSTRACT

Introduction. It was previously demonstrated by immunohistochemical analysis that there are antigens in psoriatic lesional skin that are recognized by autoantibodies in psoriatic patients and mouse antibodies to streptococcal antigens. We looked for healthy-skin-derived keratinocyte antigens recognized by those antibodies to determine whether those skin antigens are constitutive or are expressed during the development of the epidermal lesions.
Material and Methods. An extract of keratinocytes was prepared from six healthy skin biopsies. 96 microwell plates were sensitized with the keratinocyte extract to test the antibody recognition by sera from 26 psoriatic patients and 26 healthy donors, and two mice policlonal sera against total soluble extracts from S. pyogenes, with and without heat shock induction at 42°C.
Results. Healthy skin keratinocyte extract was recognized by both anti-streptococcal antisera. No significant differences were found between them. Sera from psoriatic patients did not show recognition of the antigens present in the extract of keratinocytes.
Conclusions. It seems likely that there are constitutive epidermal antigens that cross-react with S. pyogenes. Although the presence of autoantibodies that crossreact with streptococcal antigens has been reported, our results suggest that the target of those autoantibodies could be expressed during the development of disease because they are not present in the healthy skin.

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