2001, Number 3
Analysis of chromium in cattle faeces: variations in the results due to the source of faeces for standard curve calibration.
Sandoval-Castro C, Quijano-Cervera R, Ramírez-Avilés L, Cetina-Góngora R
Full text
Language: Spanish
References: 5
Page: 180-184
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ABSTRACT
Introduction. Chromiun oxide is widely used in animal nutritional studies involving digestibility, digesta flow rate and voluntary intake of ruminants. The objective of the present study was to assess the effect of different sources of cattle faeces (animals and diets) as posible sources of error in the elaboration of the chromiun standards needed in the analytical process for chromiun analysis at laboratory level. Materials and methods. Faeces, collected from four cows fed with different diets, were used to elaborate standards and calibration curves for the colorimetric analysis of chromiun. The slopes and intercepts resulting for the calibration curves were analysed statistically and the effect of the source of faeces was assessed. Results. No differences were found (p›0.05) in the slopes originated from calibration curves that included faeces from different sources. The intercepts were different between animals (p‹0.001). However, they lack of practical implications as the range of the difference was minimal (‹0.013 absorbance units). Conclusion. standards and calibration curves may be prepared using a single source of faeces without affecting the precision of the results.REFERENCES