Copes J, Nievas V, Vigo G, Sánchez M, Bagnis G, Martín V, Sanguinetti H, Perfumo CJ

Language: Spanish
References: 14
Page: 244-248
PDF size: 31.16 Kb.

ABSTRACT

Objetives. This study has investigated the isolation of Erysipelothrix rhusiopathiae from growing and fattening pigs with systemic gross lesions resembling erysipelas in La Plata Argentina. Materials and methods. Samples from tonsils, heart (endocardium), spleen, kidneys and synovial fluid with gross lesions/ changes resembling erysipelas were cultured into ESB broth and streaked onto MBA agar. Erysipelothrix rhusiopathiae strains were identified by morphological characteristics and biochemical test. Serotyping was carried out in a gel doublediffusion test with extracted antigen from the isolated strains against the specific hyperimmune sera from the 28 E. rhusiopathiae reference strains. Results. Erysipelothrix. rhusiopathiae was isolated from tonsils (without lesions), spleen (with splenomegalia), endocardium (with endocarditis) and serous fibrinous synovial exudate of two pigs and only from synovial exudate of one pig. All the isolated strains belong to se rotype 10. Primary isolation of E. rhusiopathiae was difficult because of the growth of bacteria of genus Lactobacillus spp and Enterococcus spp in both medium despite the addition of antibiotics. Discussion. Isolation of Erysipelothrix. rhusiopathiae serotype 10 associated with clinical erysipelas is relatively rare. The isolation of Erysipelothrix rhusiopathiae serotype 10 from non-cutaneous systemic lesions compatible with erysipelas in pigs constitutes the first reference of this infection in swine in Argentina. It also provide evidence of the pathogenicity of this serotype for swine.

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