González G, Savón CE, Cancio R, Valdivia A, Chacón D, Míguez J, Goyenechea A

Language: Spanish
References: 4
Page: 77-84
PDF size: 63.08 Kb.

ABSTRACT

15 strains of the respiratory sincitial virus were isolated in two outbreaks when ocurred in Habana city during the months of October to January (1994-1995) and in October 1996. The study of characterisation and analysis of the antigenic variability was carried out with a panel of 10 monoclonal antibodies which recognise lineal epitopes of the G protein. Two reference strains were used: the Long strain and the A/Mon/3/88 strain. The ELISA method was used for the tests. The results showed the variability of the G protein between the isolations and their antigenic relation with the Long prototype strain, besides confirming that all the strains circulating during those outbreaks, antigenically corresponded to the A subgroup.

REFERENCES

1. 1.- McIntosh K, Chanock R M. Respiratory syncytial virus, En Fields BN ed. Virology. New York: Raven Press, 1985. p.1285 - 304.

2. 2.- Cane PA, Pringle CR. Molecular epidemiology of respiratory syncytial virus: rapid identification of subgroup A lineages. J Virol Methods 1992; 40:297-306.

3. 3.- Anderson LJ , Hierholzer JC , Tsou C , Hendry RM ,Fernie BF , Stone Y , et al. Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. J Infect Dis 1985; 151:626-33.

4. 4.- Mufson MA, Orvell C, Rafnak B, Norrby E. Two distinct