1999, Number 2
A simple method to achieve anaerobiosis in culture media tubes.
Rodríguez-Cavallini E, Cruz E
Full text
Language: Spanish
References: 4
Page: 103-106
PDF size: 23.93 Kb.
ABSTRACT
Anaerobic bacterial cultures requires an oxigenfree atmosphere. This is why many hospital clinical laboratories only make microscopic diagnosis; in spite of the clinical importance of the anaerobes. This project proposes and evaluates a simple and economic method to prepare anaerobic culture media tubes. Material and methods. The anaerobic atmosphere within the tubes was achieved by the autoclaving and the rapid decompresion of the tubes (using rubber stoppers, needles, and screw caps). 45 species of Clostridium and 12 species of the genera Bifidobacterium, Bacteroides, Lactobacillus, Propionibacterium and Actinomyces (80 strains) were inoculated in the tubes prepared with the proposed method as well as in sterilised prereduced tubes, to evaluate growth. Results. The 80 strains that were tested, grew equally well in both pre-reduced and non prereduced media tubes, showing no difference in the degrees of turbidity. Discusion. The proposed method to achieve anaerobiosos could be a cheap and easy way to improve the culture and diagnosis of infectious anaerobic bacteria. (Rev Biomed 1999; 10:103- 106)REFERENCES