1994, Number 2
Early tolerance versus Shwartzman reaction in a mouse model of lung injury using Klebsiella pneumoniae lipopolysaccharide
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ABSTRACTA previous administration of a low dose of lipopolysacharide (LPS) induces a state of early tolerance against a further LPS inoculation enough to provoke shock and death. However, when a sublethal amount of LPS is systemically administered, after another small amount of LPS previously inoculated in the skin or other organ like the lung, a local Shwartzman reaction could occur. The objective of this study was to evaluate if a small dose of E. coli LPS administered intraperitoneally (IP), before or after a transthoracic (TT) challenge with K. pneumoniae LPS in mice, induces a benefitial or an adverse effect on the lung inflammatory response. Comparisons, employing lungwet weight as an indicator of inflammatory reaction and histopathological observations, were made. Results showed that either a previous or posterior LPS IP small dose is more phlogistic than TT induced lung lesion alone. However, only the posterior small dose of LPS IP provokes a significant aggravating effect over the previous induced TT lung lesion. This effect was considered as a Shwartzman reaction.
Alexander, H.R., Doherty, G.M., Block, M.I., Kragel, P.J., Jensen, J.C., Langstein, H.N., Walker, E. and Norton, J.A.: Single-dose tumor necrosis factor protection against endotoxin-induced shock and tissue injury in rats. Infect. Immunol., 59: 3889-3894 (1991).
Billiau, A.: Gamma-interferon: The match that lights the fire. Immunol. Today, 9: 37-40 (1988).
Henricson, B.E., Benjamin, W.R. and Vogel, S.N.: Differential cytokine induction by doses of lipopolysaccharide and monophosphoryl lipid A that result in equivalent early endotoxin tolerance. Infect. Immunol., 59: 2429-2437 (1990).
Henricson, B.E., Neta, R. and Vogel, S.N.: An interleukin-1 receptor antagonist blocks lipopolysaccharide-induced colony stimulating factor production and early endotoxin tolerance. Infect. Immunol., 59: 1188-1191 (1991).
Lynn, W.A. and Golenbock, D.T.: Lipopolysacharide antagonists. Immunol. Today, 13: 271-276 (1992).
Mori, W.: The Shwartzman reaction: A review including clinical manifestations and proposal for a univisceral of single organ third type. Histopathology, 5: 113-126 (1981).
Movat, H.Z. and Burrowes, C.E.: The local Shwartzman reaction: Endotoxin mediated inflammatory and thrombo-hemorrhagic lesions. In: Cellular Biology of Endotoxin. Edited by: Berry, L.J., 260-302. Elsevier, Amsterdam, 1985.
Ramírez, R.R., Sandoval, T.D. y Suárez, S.A.: Inducción de la reacción de Shwartzman en el pulmón del conejo empleando lipopolisacárido de Pasteurella multocida. Vet. Méx., 22: 415-424 (1991).
Rani, M., Gupta, R.K. and Chhibber, S.: Protection against Klebsiella pneumoniae induced lobar pneumonia in rats with lipopolysaccharide and related antigens. Can. J. Microbiol., 36: 885-890 (1990).
Rietschel, E.T. and Brade, H.: Bacterial endotoxins. Scientific American, 267: 26-33 (1992).
Roth, J.A.: Enhancement of nonspecific resistance to bacterial infection by biologic response modifiers. In: Virulence Mechanisms of Bacterial Pathogens. Edited by: Roth, J.A., 329-342. American Society for Microbiology, Washington, D.C., 1988.
Steel, R.G.D. and Torrie, J.H.: Principles and Procedures of Statistics. 2nd ed. McGraw-Hill, Singapore, 1981.
Stine, D.L., Huether, M.J., Moxley, R.A. and Srikumaran, S.: Actinobacillus pleuropneumoniae-induced thymic lesions in mice and pigs. Infect. Immunol., 59: 2885-2891 (1991).
Till, G.O., Morganroth, M.L., Kunkel, R. and Ward, P.A.: Activation of C5 by cobra venom factor is required in neutrophil mediated lung injury in the rat. Am. J. Pathol., 129: 44-53 (1987).
Vogel, S.N., Kaufman, E.N., Tate, M.D. and Neta, R: Recombinant interleukin-1 α and recombinant tumor necrosis factor α synergize in vivo to induce early endotoxin tolerance and associated hematopoietic changes. Infect. Immunol., 56: 2650-2657 (1988).
Zhou, W., McCollum, M.O., Levine, B.A. and Olson, M.S.: Role of platelet-activitating factor in pancreatitis-associated acute lung injury in the rat. Am. J. Pathol., 140: 971-979 (1992).
Zuckerman, S.H., Evans, G. and Butler, L.D.: Endotoxin tolerance: Independent regulation of interleukin-1 and tumor necrosis factor expression. Infect. Immunol., 59: 2774-2780 (1991).