Isolation and in situ identification of Fasciola hepatica antigens

Esteban Miranda Miranda; Zeferino García Vázquez

1994, Number 3

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Vet Mex 1994; 25 (3)

**Isolation and in situ identification of Fasciola hepatica antigens**

Miranda ME, García VZ

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Language: English/Spanish
References: 17
Page: 267-271
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ABSTRACT

Identification of antigenic sites of parasites is important to understand immunologic mechanisms and for serodiagnosis of parasitic diseases. The trematode Fasciola hepatica has a great antigenic complexity. This study was carried out to determine immunodominant antigens of 30 days of age F. hepatica by Polyacrilamide Gel Electrophoresis and Westernblot. Three antigenic polipetides (AP), with molecular weights of 24, 26 and 66 KiloDaltons (kD), were detected by Low Pressure Liquid Chromatography. Antisera against these antigens was produced in rabbits and used to identify antigenic sites on histological slides of the trematode using immunoperoxidase techniques. Specific antisera produced a dye precipitation at the following sites: 24 kD in the tegument; 26 kD in the cells of the ventral sucker and 66 kD in the intestinal cells of the parasite. It is concluded that the immunoperoxidase technique is effective and easy to perform to detect antigenic sites on histological preparations of F. hepatica and other helminths.

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