>Cirugía y Cirujanos
>Year 2009, Issue 6
Manjarrez-Gutiérrez G, Camacho-Calderón N, Mercado-Camargo R, Boyzo-Montes OA, Arvizu-Flores A, Hernández-Rodríguez J
Caracterización de células serotoninérgicas en el tejido cardiaco fetal
Cir Cir 2009; 77 (6)
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Background: We undertook this study to present biochemical and morphological characterization of serotonergic cells during fetal heart development. Methods: Wistar rats (10, 12, 16 and 20 days of gestation) were used. After obtaining the fetuses by cesarean section, the hearts were removed and fixed for immunohistochemical assay of tryptophan-5-hydroxylase (Tph), in addition to Western blot for enzyme. Serotonin concentration and Tph activity were also evaluated with high-performance liquid chromatography. Results were analyzed using Mann-Whitney U test with a significance level of p ‹0.05. Results: Metachromatic cells immunoreactive for Tph were observed from day 10 of gestation. Nerve fibers were also labeled, apparently making contact with serotonergic cells. Tph activity was measurable and serotonin levels increased with gestational age. The presence of Tph protein was confirmed by Western blot on day 16. Conclusions: The present results support the existence of cells
located in the fetal myocardium, capable of producing serotonin, whose phenotype belongs to cardiac mast cells. Their presence in this tissue strongly suggests that serotonin may play a key role in normal and abnormal development of cardiac tissue.
||Fetal heart, serotonin, tryptophan-5-hydroxylase, serotoninergic cells.
Goldmuntz E. The epidemiology and genetics of congenital heart disease.Clin Perinatol 2001;28:1-10.
Lewin MB. The genetic basis of congenital heart disease. Pediatric Ann 2000;29:469-480.
Gelb BD. Molecular genetics of congenital heart disease. Curr Opin Cardiol 1997;12:321-328.
Kirby ML. Cardiac morphogenesis: recent research advances. Pediatr Res 1987;21:219-224.
Dianne AL, Penny KA, Brian SC, Jeffrey H, Steven GH. Regulation of rat cardiac myocyte growth by a neuronal factor secreted by PC 12 cells. Pediatr Res 1997;41:832-841.
Banach K, Halbach MD, Hu P, Hescheler J, Egert U. Development of electrical activity in cardiac myocyte aggregates derived from mouse embryonic stem cell. Am J Physiol Heart Circ Physiol 2003;284:H2114-H2123.
Ikeda K, Tojo K, Otsubo Ch, Udagawa T, Kumazawa K, Ishikawa M, et al. 5-hydroxytryptamine synthesis in HL-1 cells and neonatal rat cardiocytes. Biochem Biophys Res Commun 2005;328:522-525.
Canan G, Nebigil, Maroteaux L. A novel role for serotonin in heart. TCM 2001;11:329–335.
Frishman WH, Grewall P. Serotonin and the heart. Ann Med 2000;32:195- 209.
10.Yavarone MS, Shuey DL, Tamir H, Sadler TW. Serotonin and cardiac morphogenesis in the mouse embryo. Teratology 1993;47:573-584.
11.Lauder MJ, Wilkie MB, Wu Ch, Singh S. Expression of 5-HT2B and 5-HT2c receptors in the mouse embryo. Int J Devl Neuroscience 2000;18:653-662.
Nebigil CG, Doo-Sup Ch, Dierich A, Hickel P, Le Meur M, Messaddeq N, et al. Serotonin 2B receptor is required for heart development. Proc Natl Acad Sci USA 2000;97:9508-9513.
Nebigil CG, Hickel P, Messaddeq N, Vonesch JL, Douchet MP, Monassier L, et al. Ablation of serotonin 5 HT2B receptors in mice leads to abnormal cardiac structure and function. Circulation 2001;103:2973-2985.
Nebigil CG, Etienne N, Schaerlinger B, Hickel P, Launay JM, Maroteaux L. Developmentally regulated serotonin 5-HT2B receptors. Int J Devl Neurosci 2001;19:365-372.
Choi DS, Birraux G, Launay JM, Maroteaux L. The human serotonin serotonin2B receptor: pharmacological link between serotonin2 and serotonin1D receptors. FEBS 1994;352:393-399.
Choi DS, Ward SJ, Messaddeq N, Launay JM, Maroteaux L. 5-HT2B receptor-mediated serotonin morphogenetic functions in mouse cranial neural crest and myocardiac cells. Development 1997;124:1745-1755.
Sari Y, Zhou FC. Serotonin and its transporter on proliferation of fetal heart cells. Int J Devl Neuroscience 2003;21:417-424.
Cöte F, Tévenot E, Fligny C, Fromes Y, Darmon M, Ripoche MA, et al. Disruption of the nonneuronal Tph1 gene demonstrates the importance of peripheral serotonin in cardiac function. PNS 2003;100:13525-13530.
Hansson SR, Mezey E, Hoffman BJ. Serotonin transporter messenger RNA expression in neural crest-derived structures and sensory pathways of the developing rat embryo. Neuroscience 1999;89:243-265.
Naish JS, Boenisch AJ, Farmilio U, Stead RH. Handbook of Immunochemical Staining Methods. Carpintería, CA: Dako Corp.; 1989. pp 1-41.
Johansen PA, Jennings I, Cotton RGH, Kuhn DM. Tryptophan hydroxylase is phosphorylated by protein kinase A. J Neurochem 1995;65:882-887.
Johansen PA, Jennings I, Cotton RGH, Kuhn DM. Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. J Neurochem 1996;66:817-823.
23.Peat MA, Gibb JW. High-performance liquid chromatographic determination of indoleamines, dopamine and norepinephrine in rat brain with fluorometric detection. Anal Biochem 1983;128:275-280.
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-273.
Lane D. Antibodies: A Laboratory Manual. New York: Harlow; 1988. pp. 471-509.
Galli JS. Mast cell and basophils. Curr Opin Hematol 2000;7:32-39.
Payne V, Kam PCA. Mast cell tryptase: a review of its physiology and clinical significance. Anaesthesia 2004;59:695-703.
Leon A, Buriani A, Dal Toso A, Fabris M, Romanello S, Aloe L, et al. Mast cells synthesize, store, and release nerve growth factor. Proc Natl Acad Sci USA 1994;91:3739-3743.
Palladini G, Tozzi R, Perlini S. Cardiac mast cells in the transition to heart failure: innocent bystanders or key actors? J Hypertens 2003;21:1823-1825.
Sperr WR, Bankl HC, Mundigler G, Grossschmidt AH, Simon P, Laufer P, et al. The human cardiac mast cell: localization, isolation, phenotype, and functional characterization. Blood 1994;84:3876-3884.
Bauer M, Razin E. Mast cells-nerve interactions. News Physiol Sci 2000;15:213-218.
Saxena PR, Villalon CM. Cardiovascular effects of serotonin agonists and antagonists. J Cardiovasc Pharmacol 1990;15:S17-S34.
Ramage AG. Central cardiovascular regulation and 5-hydroxytryptamine receptors. Brain Res Bull 2001;56:425-439.
McKinney J, Knappsokg PM, Haavik J. Different properties of the central and peripheral forms of human tryptophan hydroxylase. J Neurochem 2005;92:311-320.
>Cirugía y Cirujanos
>Year 2009, Issue 6