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Revista Latinoamericana de Microbiología

2008, Number 3-4

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Microbiología 2008; 50 (3-4)

Antimicrobial activity of Bothrops alternatus venom from the Northeast of Argentine

Bustillo S, Leiva LC, Merino L, Acosta O, Kier JEB, Gorodner JO

Language: English
References: 25
Page: 79-82
PDF size: 55.72 Kb.


ABSTRACT

This study was undertaken to evaluate the antimicrobial activity on Gram-negative and Gram-positive bacteria of the venom from Bothrops alternatus, specie responsible for most of snakebites in North-eastern Argentina. This activity was investigated against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), and Enterococcus faecalis (ATCC 29212). Inhibitory halos values and reduction of colony-forming unites (CFU) by dilution plate technique were determined. The bactericidal activity was higher for E. coli and S. aureus, in comparison to that for P. aeruginosa. E. faecalis was the most resistant organism tested to the action of venom solutions. The demonstrated bactericidal property of B. alternatus venom highlight it as a promising candidate for further studies according to detect which component, or components, are responsible of this activity, in order to then investigate their potential use as antimicrobial agent.


REFERENCES

  1. Acosta de Pérez, O., Koscinzuk, P., Teibler, P., Sánchez Negrete, M., Ruiz, R., Maruñak, S., Bogarín, G. 1998. Actividades hemorrágica y edematizante y alteraciones histológicas en almohadilla plantar del ratón inducidas por venenos de serpientes de los géneros Bothrops y Crotalus de Argentina. Toxicon. 36:1165-1172.

  2. Balsinde, J., Balboa, M. A., Insel, P.A., Dennis, E.A. 1999. Regulation and inhibition of phospholipase A2. Annu. Rev. Pharmacol. Toxicol. 39:175-189.

  3. Cardoso, J. L. C. 1985. Snake bites at Hospital Vital Brazil. A study of 2908 cases. Toxicon. 23:558-562.

  4. De Lima, C. D., Alvarez, A. P., Freitas, C., Oliveira, S. D., Oliveira, RS., Dos Santos, C., Mendes Cabral, L., Rodrigues, C. R., Castro, H. C. 2005. Snake Venom: Any Clue for Antibiotics and CAM? eCAM. 2(1):39-47.

  5. Gutiérrez, J. M. 1995. Clinical toxicology of snakebite in Central America. In: Meier, J., White, J. (Eds.). Handbook of Clinical Toxicology of Animal Venoms and Poisons. CRC Press, Boca Raton, 645-665.

  6. Hancock, R. E. and Chapple, D. Peptide antibiotics. 1999. Antimicrob. Agents Chemother. 43:1317-1323.

  7. Heise, P.J., Maxson, L.R., Dowling, H.G., Hedges, S.B. 1995. Higher level snake phylogeny inferred from mitochondrial DNA sequences of 12S rRNA genes. Mol. Biol. Evol. 12:259-265.

  8. Izidoro, L. F. M, Ribeiro, M. C., Souza, G. R. L., Sant’Ana, C. D., Hamaguchi, A., Homsi-Brandeburgo, M.I., Goulart, L. R., Beleboni, R. O., Nomizo, A., Sampaio, S. V., Soares, A. M., Rodrigues, V. M. 2006. Biochemical and functional characterization of an L-aminoacid oxidase isolated from Bothrops pirajai snake venom. Bioorg. Med. Chem. 14 (20):7034-7043.

  9. Lomonte, B., Pizarro-Cerda, J., Ângulo, Y., Gorvel, J. P., Moreno, E. 1999a. Tyr-Trp-substituted peptide 115-129 of a Lys49 phospholipase A2 expresses enhanced membrane-damaging activities and reproduces its in vivo myotoxic effect. Biochim Biophys Acta. 1461:19-26.

  10. Lomonte, B., Angulo, Y., Rufini, S., Cho, W., Giglio, J. R., Ohno, M., Daniele, J. J., Geoghegan, P., Gutierrez, J. M. 1999b. Comparative study of the cytolytic activity of myotoxic phospholipases A2 on mouse endothelial (tEnd) and skeletal muscle (C2C12) cells in vitro. Toxicon. 37:145-58.

  11. Markland, F. S. Jr. 1997. Snake venoms. Drugs. 54, (3):1–10

  12. Mazzi, M. V., Marcussi, S., Carlos, G. B., Stabeli, R. G., Franco, J. J., Ticli, F. K., Cintra, A. C. O., Franca, S. C., Soares, A. M., Sampaio S V. 2004. A new hemorrhagic metalloprotease from Bothrops jararacussu snake venom: isolation and biochemical characterization. Toxicon. 44, 2:215-223.

  13. Meier, J. and Stocker, K. 1991. Effects of snake venoms on hemostasis. Crit. Rev. Toxicol. 21:171-82.

  14. Páramo, L., Lomonte, B., Pizarro-Cerda, J., Bengoechea, J. A., Gorvel, J., Moreno, E. 1998. Bactericidal activity of Lys49 and Asp49 myotoxic phospholipases A2 from Bothrops asper snake venom. Synthetic Lys49 myotoxin II-(115-129)-peptide identifies its bactericidal region. Eur. J. Biochem. 253:452-461.

  15. Roberto, P. G., Kashima, S., Marcussi, S., Pereira, J., Astol.-Filho, S., Nomizo, A., Giglio, J. R., Fontes, M. R. M., Soares, A. M., and França, S.C. 2004. Cloning and Identification of a Complete cDNA Coding for a Bactericidal and antitumoral acidic phospholipase A2 from Bothrops jararacussu Venom. Protein J. 23, 4: 273-285.

  16. Rodríguez, V. M., Marcussi, S., Cambraiab, R. S., Araújob, A. L., Malta-Netob, N. R., Hamaguchia, A., Ferroc, E. A. V., Homsi-Brandeburgoa, M. I., Gigliod, J. R. and Soares, A. M. 2004. Bactericidal and neurotoxic activities of two myotoxic phospholipases A2 from Bothrops neuwiedi pauloensis snake venom. Toxicon, 44, 3 , 1:305-314.

  17. Russell, F. E. 1980. Snake Venom Poisoning. Philadelphia: J. B. Lippincott.

  18. Santamaría, C., Larios, S., Quiro´s, S., Pizarro-Cerda, J., Gorvel, J. 2004. Bactericidal and Antiendotoxic Properties of Short Cationic Peptides derived from a Snake Venom Lys49 Phospholipase A2. Antimicrob. Agents Chemother. 49, 4:1340-1345.

  19. Santamaría, C., Larios, S., Angulo, Y., Pizarro-Cerda, J., Gorvel, J., Moreno, E. Lomonte, B. 2005. Antimicrobial activity of myotoxic phospholipases A2 from crotalid snake venoms and synthetic peptide variants derived from their C-terminal region. Toxicon. 45, 7 , 1:807-815.

  20. Soares, A. M., Guerra-Sa, R., Borja-Oliveira, C., Rodrigues, V. M., Rodrigues-Simioni, L., Rodrigues, V., Fontes, M., Lomonte, B. 2000. Structural and Functional Characterization of BnSP-7, a Lys49 Myotoxic Phospholipase A2 Homologue from Bothrops neuwiedi pauloensis Venom. Arch. Biochem. Biophys. 378, 2:201–209.

  21. Soares, A. M., Mancin, A. C., Cecchini, A. L., Arantes, E. C., França, S. C., Gutiérrez, J. M., Giglio, J. R. 2001. Effects of chemical modifications of crotoxin B, the phospholipase A2 subunit of crotoxin from Crotalus durissus terrificus snake venom, on its enzymatic and pharmacological activities. Int J Biochem Cell Biol. 33, 9:877-888.

  22. Stabeli, R. G., Marcussi, S., Guilherme, B. C., Rosemeire, C. L. R. Pietro, H. S. 2004. Platelet aggregation and antibacterial effects of an L-amino acid oxidase purified from Bothrops alternatus snake venom. Bioor Med Chem. 12:2881-2886.

  23. Tashima, A. K., Sanz, L., Camargo, A. C. M., Serrano, S. M. T., Calvete, J. J. 2008. Snake venomics of the Brazilian pitvipers Bothrops cotiara and Bothrops fonsecai. Identification of taxonomy markers. Journal of proteomics. 71: 473-485.

  24. Toyama, M. H., Garcia de Oliveira, D., Beriam, L. O. S., Camillo Novello, J., Rodrigues-Simioni, L., Marangoni, S. 2003. Structural, enzymatic and biological properties of new PLA2 isoform from Crotalus durissus terrificus venom. Toxicon, 41:1033-1038.

  25. Vogel, C. W., Fritzinger, D. C., Hew, B. E., Thorne, M., Bammert, H. 2004. Recombinant cobra venom factor. Mol Immunol. 41: 191-199.




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