Martínez HE, Esteves JA, Tenorio BI, Arroyo ES, Moncada BD, Arenas GR

Language: Spanish
References: 9
Page: 338-341
PDF size: 194.66 Kb.

ABSTRACT

Background: The hemoculture confirms a bacteremia. The isolated microorganisms in hospitals’ rooms are one of the most important causes of morbidity and mortality.
Objective: To determine the frequency of microbiological isolated agents in hemocultures in pediatric and adult patients hospitalized at Hospital General Dr. Manuel Gea Gonzalez.
Materials and methods: We reviewed all hospitalized patients’ records with positive hemocultures from June 2005 to May 2007. We classified as positive samples those identified after 1-7 days of incubation in automated equipment BACTEC 9120 (BECKTON Dickinson^®). We performed in all of them a Gram stain, culture in selective media, and biochemical specific tests.
Results: Of 4,381 hemocultures registered, 533 (12.7%) were positive: 72 (13.5%) in newborns, 170 (31.9%) in children (1 month to 18 years) and 291 (54.6%) in adults. In 302 samples (56.7%) were identified grampositive microorganisms (Staphylococcus negative coagulase, Staphylococcus aureus, others.), 200 (37.5%) gramnegative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, etc.), and 31 (5.8%) yeast (Candida spp and others). In Pediatrics, 42.2% of the isolates were obtained, 38.8% in Internal Medicine, 26.5% in Neonatal Intensive Care Unit, 14.1% and 12.8% in Surgery in the Intensive Care Unit.
Discussion: Hemoculture isolated microorganisms of different room’s patients can have different origin or contamination source
Conclusions: Frequency of microbiological isolation in hemocultures was 12.7%; most frequently isolated agents were gram-positive microorganisms.

REFERENCES

1. Bilal NE, Gedebou M, Al-Ghamdi S. Endemic nosocomial infections and misuse of antibiotics in a maternity hospital in Saudi Arabia. APMIS 2002;110:140-7.

2. Weisntein MP, Towns ML, Quartey SM, Mirret S, et al. The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology and outcome of bacteremia and fungemia in adults. Clin Infect Dis 1997;24:584-602.

3. Guillemont D, Gasquet I, Vallet O, David MF, et al. Thirty-day mortality of nosocomial systemic bacterial infections according to antibiotic susceptibility in an 800-bed teaching hospital in France. Clin Microbiol infect 2005;11(6):502-4.

4. Gillespie TA, Johnson PRE, Notman AW, Coia JE, Hanson MF. Eradication of a resistant Pseudomonas aeruginosa strain after a cluster of infections in a hematology/oncology unit. Clin Microbiol Infect 2000;6:125-30.

5. Ferguson JK, Gill A. Risk-stratified nosocomial infection surveillance in a neonatal intensive care unit: report on 24 months of surveillance. J Paediatr Child Health 1996;32:525-31.

6. Chávez-García A, Cabrera-Rayo A, Marín-Romero MC, Villagómez- Ortiz A y col. Incidencia y pronóstico de candidiasis invasora en pacientes no neutropénicos de terapia intensiva. Med Int Mex 2007;23(6):481-5.

7. Park CH, Seo JH, Lim JY, Woo HO, Youn HS. Changing trend of neonatal infection: experience at a newly established regional medical center in Korea. Pediat Inter 2007;49:24-30.

8. López DML, Martinez JA, Vidal M, Almela M, et al. Clinical characterization of breakthrough bacteraemia, a survey of 392 episodes. J Inter Med 2005;258:172-80.

9. Esel D, Doganay M, Alp E, Sumerkan B. Prospective evaluation of blood cultures in a Turkish university hospital: epidemiology, microbiology and patient outcome. Clin Microbiol Infect 2003;9:1038-44.