2008, Number 6
IL-6 seric determination and PCR qualitative test in subjects with herpes simplex virus positive IgM
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ABSTRACTBackground: Herpes simplex virus (HSV-1 and HSV-2) infection can remain in latent state and re-activate itself with or without clinical symptoms; due to this, several laboratory tests should be done to detect this type of infection.
Objective: To determine seric levels of interleukin-6 (IL-6) and made PCR probe in subjects with IgM positive to HSV.
Material and methods: Eighteen serum samples from subjects with IgM positive for herpes simplex virus (HSV-1 and HSV-2) were analyzed. Serum levels of IL-6 were determined by ELISA test and a qualitative PCR test for HSV detection was carried out. Control group has 20 seemingly healthy subjects with negative levels of immunoglobulins for HSV.
Results: IgM index in serum of subjects with HSV was 1.767 ± 0. 748 and all had negative PCR test. In comparison to control group, group with HSV had a high seric concentration of IL-6 (36.11 ± 5.27 vs 121.9 ± 23.91 pg/mL; p ‹ 0.001; respectively). IL-6 shows a positive correlation with seric levels of IgM (r = 0.668; p ‹ 0.0024).
Conclusion: Negative result in PCR test of all subjects with high serum levels of IgM for HSV indicates the presence of an infection without circulating virus. On the other hand, high levels of IL-6 in this type of subjects show the presence of an inflammatory process with an important correlation with the IgM levels in serum. All at once indicates an infectious process caused by this type of virus. All at once indicates an infectious process caused by this type of virus.
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