2005, Number 73
Controlled evaluation of the necessary blood volume for the detection of bacteriemia or funguemia in newborns
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ABSTRACTIntroduction: A lot volume of blood is extracted from the newborn for diagnostic purposes. Including blood samples for cultures with subsequent complications like anemia. However, the daily use of blood cultures to sepsis diagnosis, the volume values to detect bacteremia or funguemia in newborns are not available.
Material and methods: In a in-vitro assay, we selected six more common pathogens of neonatal sepsis as E. coli, S. aureus, S. epidermidis, Klebsiella pneumoniae, E. faecalis and Candida albicans, latterly we inoculated into sterile blood the microorganisms in two dilutions, the first with 10 UFC/ml. and the second one with 1 UFC/ml. To each microorganism we inoculated 4 bottles (Pedi-Bact / Alert) and subsequently were incubated for seven days into an automated detection system.
Results: We inoculated 24 bottles, with positives results to microorganism’s growth into a mean time of 16.5 ± 7.3 hours, E. coli and K. pneumoniae were the faster microorganisms to growth with 11.5 and 10.3 hours in mean respectly. C. albicans was the microorganism slower to growth with 26.3 hours.
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