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Revista Mexicana de Periodontología

ISSN 2007-4360 (Print)
Asociación Mexicana de Periodontología, Colegio de Periodoncistas A.C
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2010, Number 1

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Rev Mex Periodontol 2010; 1 (1)

Bio-modification of the root surfaces treated with tretraacetic etildiamino acid (EDTA) in surgical periodontal treatment: Structural analysis

Guerrero ÁF, Morales HD, Téllez JH, Todd JM
Full text How to cite this article

Language: Spanish
References: 5
Page: 19-22
PDF size: 375.18 Kb.


Key words:

Root surfaces, periodontal treatment, ultrastructural analysis.

ABSTRACT

Evaluate scanning electron microscopy demineralization of root surfaces treated with EDTA and observe their biomodification. Introduction: Root debridement generates a layer of waste containing microorganisms and toxins that could interfere with periodontal healing. For this reason, different substances have been used to eliminate it and to expose collagen fibers on the surface of the tooth. Ethylenediaminetetraacetic acid (EDTA) as a gel to 24% with neutral pH, is a root biomodificator which proposes that when applied to root surfaces, acts as a chelating agent that selectively remove hydroxyapatite matrix collagen from the tooth surface, allowing periodontal ligament cells proliferate more rapidly on it, reinforcing the unity of this area with new gingival fibroblasts. Methodology: 30 bodies were selected tooth indicated for extraction divided into two groups, there was scraping one of them is placed 24% EDTA gel at neutral pH, at the end of 2 min and washed with saline. There are repositioned in the alveoli and impregnated with fresh blood for 3 min, and dried, fixed with 37% formalin and taken to ultrastructural analysis. The photomicrographs were obtained at 500x, 1,000x, 1,500x. Result: The dental organs of the control group and handled with EDTA, there were wide variations in morphological aspects. In the control group, the root surface shows irregular and rough, with a layer of waste which caused the obliteration of the dentinal tubules and demineralized. In the group treated with 24% EDTA gel shows a clean surface with great exposure demineralized dentin tubules. Conclusions: The use of EDTA gel is effective in decontamination and demineralization of the root surface which provides a substrate for a new attachment of connective tissue in periodontal surgical treatment.


REFERENCES

  1. Manzolli F et al. Blood cell attachment to root surfaces treated with EDTA gel. Braz Oral Res 2005; 19 (2): 88-92.

  2. Shetty B, Dinesh A, Seshan H. Comparative effects of tetracyclines and citric acid on dentin root surface of periodontally involved human teeth: A scanning electron microscope study. J Indian Soc Periodontol 2008; 12: 8-15.

  3. Babay N. The effect of EDTA on the attachment and growth of cultured human gingival fibroblasts in periodontitis-affected root surface. J. Contemp Dent Pract 2001; 2 (1): 13-23.

  4. Wennstrom JL, Lindhe J. Some effects of enamel matrix proteins on wound healing in the dento-gingival region. J Clin Periodontol 2002; 29 (1): 9-14.

  5. Smith BA, Smith JS, Caffesse RG, Nasjteti CE, Lopatin DE, Kowalski CJ. Effect of citric acid and various concentrations of fibronectin on healing following periodontal flap surgery in dogs. J Periodontol 1987; 58: 667-73.




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Rev Mex Periodontol. 2010;1