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2010, Number 3

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Enf Infec Microbiol 2010; 30 (3)

Sensitivity and specificity of two Chromogenic media for Candida spp identification

Cortés FAÁ, Aguilar SA, Carrera MO, Juárez S, Bazán ME, Castañón OLR

Language: Spanish
References: 13
Page: 78-82
PDF size: 139.04 Kb.


ABSTRACT

BACKGROUND. Mycosis in Mexico have increased significantly its incidence and the Candida genus is one of the main causes. Species identification by chromogenic media CHROMagarTMCandida and ID2TMCandida is common in the literature and various reports are advising about its use. The aim of this study is to know the efficiency of each of these two culture media for Candida species identification.
MATERIAL AND METHOD. From patients of Centro Medico Nacional 20 de Noviembre, ISSSTE, were obtained 100 Candida spp sample, which were cultured on chromogenic media CHROMagarTMCandida and ID2TMCandida. Simultaneously, the sample were subjected to automated auxanogram (VITEK®), and the results were the reference for calculating sensitivity and specificity for each chromogenic medium.
RESULTS. Respectively, sensitivity and specificity shown for C. albicans was 94% and 81% for CHROMagarTMCandida, and 90% and 70% in ID2TMCandida.
CONCLUSIONS. For the sensitivity, both chromogenic media are useful for identifying C. albicans, but due to poor specificity that shown, are not recommended to identify some defined Candida non albicans, because of which it becomes necessary to use other methods.


REFERENCES

  1. Calderone RA. Candida and Candidiasis. Washington, of the new chromogenic medium Candida ID2 for iso-DC: American Society Microbiolgy Press, 2001: 451 pp. lation and identification of Candida Albicans and other.

  2. González GM, Elizondo M, Ayala J. “Trends in species medically important Candida species”. J Clin Microb distribution and susceptibility of blood stream isolates 2006; 44: 3340-3345. of Candida collected in Monterrey, Mexico, to seven 8. Yucesoy M, Ozbek OA, Marol S. “Comparison of three antifungal agents: Result of 3-Year (2004-2007) survei-differential media for the presumptive identification of llance study”. J Clin Microb 2008; 46: 2902-2905. yeasts”. Clin Microbiol Infect 2005; 11: 245-247.

  3. Guilarte C, Pardi G. “Pruebas para identificar especies 9. Ballesté R, Arteta Z, Fernández N, Mier C, Mousqués de Candida en cavidad bucal”. Acta Odonto Venezolana N, Xavier B, Cabrera MJ, Acosta G, Combol A, Gezuele 2009; 47: 1-7. E. “Evaluación del medio cromógeno CHROMagar

  4. Sánchez Flores MA. Relación de Candida spp. obtenida CandidaTM para la identificación de levaduras de interés de pacientes y su ambiente en la unidad de cuidados médico”. Rev Med Uruguay 2005; 21: 186-193. intensivos de adultos (UCIA) del Centro Médico Nacio-10. Yücesoy M, Marol S. “Performance of CHROMagar nal (CMN) 20 de noviembre, ISSSTE. Tesis de la carrera Candida and BIGGY Agar for identification of yeast de Biología. México, DF. Facultad de Ciencias, UNAM, species”. Ann Clin Microb Antimicrob 2003; 2: 8. 2009: 59 pp. 11. García-Martos P, García-Agudo R, Hernández-Molina

  5. Sendid B, François N, Standaert A, Dehecq E, Zerimech F, JM, Marín P, Tallero E, Mira J. “Identificación de levadu-Camus D, Poulain D. “Prospective evaluation of the new ras de interés clínico en el medio de cultivo CHROMa-chromogenic medium candiselect 4 for differentiation garTM Candida”. Rev Iberoam Micol 1998; 15: 131-135. and presumptive identification of the major pathogenic 12. Lestcher-Bru V, Meyer MH, Galoisy AC, Waller J, Candol-Candida species”. J Med Microb 2007; 56: 495-499. fi E. “Prospective evaluation of the new chromogenic

  6. Peng CF, Lee KM, Lee SH. “Characterization of two medium Candida ID in comparison with candiselect, chromogenic media of Candida ID2 and Chromagar for isolation of molds and isolation and presumptive Candida for preliminary identification of yeast”. J Bio-identification of yeast species”. J Clin Microb 2002; 40: medic Lab 2006; 19: 63-68. 1508-1510.

  7. Eraso E, Moragues MD, Villar-Vidal M, Sahand IH, 13. Fletcher R. Epidemiologia clínica. 4a edición. Lippincott González-Gómez N, Pontón J, Quindós G. “Evaluation Williams & Wilkins. Wolters Kluwer Health, 2008: 120-237.

  8. Yucesoy M, Ozbek OA, Marol S. “Comparison of three differential media for the presumptive identification of yeasts”. Clin Microbiol Infect 2005; 11: 245-247.

  9. Ballesté R, Arteta Z, Fernández N, Mier C, Mousqués N, Xavier B, Cabrera MJ, Acosta G, Combol A, Gezuele E. “Evaluación del medio cromógeno CHROMagar CandidaTM para la identificación de levaduras de interés médico”. Rev Med Uruguay 2005; 21: 186-193.

  10. Yücesoy M, Marol S. “Performance of CHROMagar Candida and BIGGY Agar for identification of yeast species”. Ann Clin Microb Antimicrob 2003; 2: 8.

  11. García-Martos P, García-Agudo R, Hernández-Molina JM, Marín P, Tallero E, Mira J. “Identificación de levadu- ras de interés clínico en el medio de cultivo CHROMa- garTM Candida”. Rev Iberoam Micol 1998; 15: 131-135.

  12. Lestcher-Bru V, Meyer MH, Galoisy AC, Waller J, Candol- fi E. “Prospective evaluation of the new chromogenic medium Candida ID in comparison with candiselect, for isolation of molds and isolation and presumptive identification of yeast species”. J Clin Microb 2002; 40: 1508-1510.

  13. Fletcher R. Epidemiologia clínica. 4a edición. Lippincott Williams & Wilkins. Wolters Kluwer Health, 2008: 120-237.




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