2009, Number 5
Rev Mex Urol 2009; 69 (5)
3-nitrotyrosine (3-NT) determination in tissues with prostate cancer and benign prostatic hyperplasia
Floriano-Sánchez E, Cárdenas-Rodríguez N, Castro-Marín M, Zapata-Villalba MA, Flores-Terraza JE, Torres-Salazar JJ
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ABSTRACTRecent reports have placed prostate cancer (CaP) as the third cause of death by cancer in the world and as the principal cause in Mexico after skin cancer. The role of oxidative stress has been observed in cancer etiology. Due to the elevated incidence of prostate cancer in the Mexican population, it is of vital importance to contribute to the development of new hypotheses resulting in more complex studies and in this way increasing the probability of developing molecular markers for diagnosing cancer.
Objective: To evaluate the existence of any alteration in levels of 3-nitrotyrosine (3-NT), an oxidative stress marker, in prostate cancer and benign prostatic hyperplasia.
Materials and methods: Thirty benign prostatic hyperplasia tissue samples and forty prostate cancer tissue samples were obtained and conditions were standardized to immunohistochemically detect the presence of 3-nitrotyrosine in tissue.
Results: The percentages of immunoreactive 3-nitrotyrosine were 25.78% in prostate cancer tissue and 4.43% in benign prostatic hyperplasia tissue.
Conclusions: Densitometric data of 3-NT immunoreactivity in prostate cancer tissue were higher than those obtained in benign prostatic hyperplasia tissue, suggesting that 3-NT determination could be used as a tumor marker.