2011, Number 1
Synovial sarcoma. clinicopathologic and immunohistochemical study of 21 cases with emphasis on TLE-1, p16 and cyclin D-1
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ABSTRACTBackground: Synovial sarcoma is a malignant neoplasm with specific clinical, morphological and genetic characteristics.
Objective: To report the clinicopathological and immunohistochemical analysis of 21 cases of synovial sarcoma, specially the expression of three markers by immunohistochemistry: TLE-1, cyclin and p16.
Material and method: A clinicopathologic and immnohistochemical study was done of 21 cases of synovial sarcomas (three biphasic, 16 monophasic fibrous and two poorly differentiated) using, among other markers, TLE-1, p16 and cyclin D1.
Results: TLE-1 antibody showed a positive nuclear stain in 62% (13/21) of the cases. p16 antibody revealed a diffuse positive nuclear staining in 14% (3/21), heterogeneous expression in 48% (10/21) and negative staining in 38% (8/21). Cyclin D1 showed increased expression in 71% (15/21), low expression in 19% (4/21), and negative staining in 10% (2/21).
Conclusions: TLE-1 is a useful adjunct immunohistochemical marker in combination with the classic (EMA, keratin and Bcl-2) markers of synovial sarcoma. The expression of p16 and cyclin D1 have been correlated with the previously described genetic alteration of the cell cycle deregulations in synovial sarcoma.
Subramaniam MM, Navarro S, Pellin A, López Guerrero JA, et al. Tissue microarrays profiling of primary and xenotransplanted synovial sarcoma demonstrates the immunophenotypic similarities existing between SYT-SSX fusion gene confirmed, biphasic, and monophasic fibrous variants. Virchows Arch 2006;449:435-447.