2011, Number 2
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ABSTRACTBackgrond: Inflammatory stimuli have been found to exert little effect on COX-1 expression, but are well-known for leading to a rapid rise in COX-2 mRNA, suggesting that COX-2 plays a role in the inflammation process. COX-2 may also play an important role in neurodegeneration,and in particular in citotoxic neuron death.
Objective: To analyze in brain tissue from autopsies of 13 patients with cancer –five of them treated with chemotherapy and the other eight without chemotherapy– expression of cyclooxygenase-2 (COX-2), mRNA and interleukin-1β.
Material and methods: In brain tissue of 13 patients with different kinds of primary malignant tumors the interleukin-1β and cyclooxygenase-2 expression was analyzed in autopsy, five patients were treated with chemotherapy and eight without chemotherapy. It was realized by real time RT-PCR of total RNA of cerebral cortex and white materia tissue preserved in -80°C, and it was used, primers and fluorophore-labeled TaqMan probes targeting human COX-2 and IL-1β.
Results: Brain tissue from patients with chemotherapy had significantly higher interleukin-1β and cyclooxygenase-2 expression in frontal cortex and hippocampus.
Conclusion: These findings suggest that exposure to cytotoxic drugs is associated with COX-2 gene induction by interleukin-1β, neuronal dysfunction that could precede the neurologic complications of chemotherapy treatment.
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