2011, Number 4
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ABSTRACTObjective: To investigate the effects of the ultraviolet (UV) irradiation of titanium (Ti) plates on osteoblastic adhesion using a simple and reproducible colorimetric method to determine cell density.
Material and methods: Two different sizes of (n=10 each) plates (10 x 10 x 0.5 mm and 20 x 20 x 0.05 mm) were obtained from a sheet of pure titanium, which were then divided into two groups (n=5/gp) for each. The surface of the Ti plates was polished before being examined under a scanning electron microscope (SEM) to estimate the roughness of the polished surface. The group of experimental Ti plates was UV irradiated at a wavelength of 253.7 nm for 5, 20, 40, 60 minutes, or 4 and 6 hours. MC3T3-E1 mouse osteoblasts were cultured in an alpha-minimum essential medium (α-MEM) and inoculated on each Ti plate. The number of adherent and proliferated cells was determined using the the MTT method and amino acid consumption.
Results: As little as 20 minutes of UV radiation of the Ti plates significantly (p‹0.05) improved the adhesion and proliferation of osteoblasts, and resulted in an increased consumption of glutamine and arginine.
Conclusions: UV irradiation of Ti plates significantly enhanced osteoblastic adhesion using the MTT method, confirming the potential of UV light
Buser D, Broggin N, Wieland M, Scheken RK, Denzar AJ, Cochran DL, Hoffman B, Lussini A, Steinemann SG. Enhanced bone opposition to a chemically modified SLA titanium surface. J Dent Res 2004; 83: 529-533.
Onuki H, Sakagami H, Kobayashi M, Hibino Y, Yokote, Nakajima H, Shimada J. Effect of contact with titanium alloys on the proliferation of mouse osteoblastic cells in culture. In Vivo 2010; 24: 29-37.
Yang GL, He FM, Hu JA, Wang XX, Zhao SF. Effects of biomimetically and electrochemically deposited nano-hidroxiapatie coating on osseointegration of porous titanium implants. Oral Surg Oral Med Oral Phatol Oral Radio Endod 2009; 107: 782-789.
Iwaya Y, Machigashira M, Kanbara K, Miyamoto M, Noguchi K, Izumi Y, Ban S. Surface properties and biocompatibility of acidetched titanium. Dent Mater J 2008; 27: 413-421.
Kim KH, Ramaswamy N. Electrochemical surface modification of titanium in dentistry. Dent Mater J 2009; 28: 20-36.
Chiesa R, Giavaresi G, Fini M, Sandrini E, Giordano C, Bianchi A, Giardino R. In vitro and in vivo performance of a novel surface treatment to enhance osseointegration of endosseous implants. Oral Surg Oral Med Oral Phatol Oral Radio Endod 2007; 103: 745-756.
Albresktsson T, Zarb G, Worthington P, Eriksson A. The long-term efficacy of currently used dental implants. A review and proposed criteria of success. Int J Oral Maxillofac Implants 1986; 1: 11-25.
Hartman LC, Meenaghnan MA, Schaaf NG, Howker PB. Effects of pretreatment sterilization and cleaning methods on materials properties and osseoinductivity of threaded implants. Int J Oral Maxillofac Implants 1989; 4: 11-18.
Riley DJ, Bavastrello V, Covani U, Barone A, Nicolini C. An in-vitro study of the sterilization of titanium dental implants using low intensity UV-radiation. Dent Mater 2005; 21: 756-760.
Singh S, Schaaf N. Dynamic sterilization of titanium implants with ultraviolet light. Inter J Oral Maxillo 1989; 4: 139-146.
Wang R, Hashimoto K, Fujishima A. Light-indiced amphiphilic surfaces. Nature 1997; 388: 431-432.
Tengvall P, Lundström I. Physico-chemical considerations of titanium as a biomaterial. Clin Mater 1992; 9: 115-134.
Lahann J, Mitragotri S, Tran TN, Kaido H, Sundaram J, Choi IS, Hoffer S, Samorjai GA, Langer R. A reversibly switching surface. Science 2003; 299: 371-374.
Kieswetter K, Schwartz Z, Dean DD, Boyan BD. The role of implant surface characteristics in the healing of bone. Crit Rev Oral Biol Ued 1996; 3: 329-245.
MacDonald DE, Deo N, Markovic B, Stranick M, Somasundaran P. Adsorption and dissolution behavior of human plasma fibronectin on thermally and chemically modified titanium dioxide particles. Biomaterials 2002; 23: 1269-1270.
Zhao G, Schawarts Z, Wieland M, Rupp F, Geis-Gerstorfer J, Cochran DL, Boyan BD. High surface energy enhances cell response to titanium substrate microstructure. J Biomed Mater Res A 2005; 74: 49-58.
Yamamichi N, Pugdee K, Chang W, Lee S, Yoshinari M. Gene expression monitoring in osteoblast on titanium coated with fibronectin-derived peptide. Dent Mater J 2008; 27: 744-750.
Makihira S, Mine Y, Kosaka E, Nikawa H. Titanium surface roughness accelerates RANKL-dependent differentiation in the osteoclast precursor cell line, RAW264.7. Dent Mater J 2007; 26: 739-745.
Ueno T, Yamada M, Suzuki T, Minamikawa H, Sato N, Hori N, Takeuchi K, Hottoti M, Ogawa T. Enhancement of bone-titanium integration profile with UV photofunctionalized titanium in a gap healing model. Biomaterials 2010; 31: 1546-1557.
Att W, Hori N, Iwasa F, Yamda M, Ueno T, Ogawa. The effect of UV-photofunctionalization on the time-related bioactivity of titanium and chromium-cobalt alloys. Biomaterials 2009; 30: 4268-4276.
Aita H, Att W, Ueno T, Yamada M, Hori N, Iwasa F, Tsukimura N, Ogawa T. Ultraviolet light-mediated photofunctionalization of titanium to promote human mesenchymal stem cell migration, attachment, proliferation and differentiation. Acta Biomater 2009; 5: 3247-3257.
Sawase T, Jimbo R, Baba K, Shibata Y, Ikeda T, Atsuta M. Photoinduced hydrophilicity enhances initial cell behavior and early bone apposition. Clin Oral Impl Res 2008; 19: 491-496.
Zhu Y, Watari F. Surface carbonization of titanium for abrasion resistant implant materials. Dent Mater J 2007; 26: 245-253.
Tamura Y, Yokoyama A, Watari F, Kawasaki. Surface properties and biocompatibility of nitrited titanium for abrasion resistant implant materials. Dent Mater J 2002; 21: 355-372.
Okawa S, Watanabe K. Chemical polishing of titanium with colloidal silica containing hydrogen peroxide-mirror polishing and surface properties. Dent Mater J 2009; 2: 68-74.
International Organization for Standardization: Geometrical Product Specifications (GPS): Surface texture, Profile method: Terms, definitions and surface texture parameters. ISO 4287: 1997.
Sudo H, Kodoma H, Amagai Y, Yamamoto S, Kasai S. In vitro differentiation and calcification in new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol 1983; 96: 191-198.
Sakagami H, Kishino K, Amano O, Kanda Y, Kunii S, Yokote Y, Oizumi H. Oisumi T. Cell death induced by nutritional starvation in mouse macrophage-like RAW264.7 cells. Anticancer Res 2009; 29: 343-348.
Fujishima A and Honda K. Electrochemical photolysis of water at a semiconductor electrode. Nature 238: 37-38, 1972.
Xu MF, Lin S, Chen XM and Peng YZ. Studies on characteristics of nanostructure of N-TiO2 thin films and photobactericidal action. J Zhejiang Univ Sci B 7: 586-590, 2006.
Koseki H, Shiraishi K, Asahara T, Tsurumoto T, Shindo H, Baba K, Taoda H and Terasaki N. Photocatalytic bactericidal action of fluorescent light in a titanium dioxide particles mixture: an in vitro study. Biomed Res 30: 189-192, 2009.
Susuki T, Hori N, Att W, Kubo K, Iwasa F, Ueno T, Maeda H and Ogawa T. Ultraviolet treatment overcomes time-related Degrading Bioactivity of titanium. Tissue Engineering 2009; 15:3679-3888.
Iwasa F, Hori N, Minamikawa H and Ogawa T: Enhancement of osteoblast adhesion to UV-photofunctionalized titanium via an electrostatic mechanism. Biomaterials 2010; 31: 2717-2727.
Bico J, Tordeux C and Quérés D. Rough wetting. Europhys Lett 2001; 55: 214-220.