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2011, Number 4

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Rev ADM 2011; 68 (4)

The effects of the ultraviolet irradiation of titanium plates on osteoblastic adhesion

García-Contreras R, Scougall-Vilchis RJ, Contreras-Bulnes R, Adachi K, Sakagami H, Hibino Y, Nakajima H, Shimada J

Language: Spanish
References: 34
Page: 175-182
PDF size: 218.65 Kb.


ABSTRACT

Objective: To investigate the effects of the ultraviolet (UV) irradiation of titanium (Ti) plates on osteoblastic adhesion using a simple and reproducible colorimetric method to determine cell density.
Material and methods: Two different sizes of (n=10 each) plates (10 x 10 x 0.5 mm and 20 x 20 x 0.05 mm) were obtained from a sheet of pure titanium, which were then divided into two groups (n=5/gp) for each. The surface of the Ti plates was polished before being examined under a scanning electron microscope (SEM) to estimate the roughness of the polished surface. The group of experimental Ti plates was UV irradiated at a wavelength of 253.7 nm for 5, 20, 40, 60 minutes, or 4 and 6 hours. MC3T3-E1 mouse osteoblasts were cultured in an alpha-minimum essential medium (α-MEM) and inoculated on each Ti plate. The number of adherent and proliferated cells was determined using the the MTT method and amino acid consumption.
Results: As little as 20 minutes of UV radiation of the Ti plates significantly (p‹0.05) improved the adhesion and proliferation of osteoblasts, and resulted in an increased consumption of glutamine and arginine.
Conclusions: UV irradiation of Ti plates significantly enhanced osteoblastic adhesion using the MTT method, confirming the potential of UV light


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