Curiel HH, Vélez CH, Lora GJ

Language: Spanish
References: 12
Page: 423-430
PDF size: 145.48 Kb.

ABSTRACT

Mangiferin was selected as marker in the quality control of active principle VIMANG® and its formulations, which have been used as nutritional supplement, cosmetic, and phytomedication. This paper was aimed at validating the capillary electrophoresis method for the quantitative analysis of mangiferin in the pharmaceutical formulation 300 mg VIMANG® tablets. The specificity, linearity, accuracy and precision were assessed. The results showed the method specificity owing to lack of interference by the excipients or their degradation products. Linearity showed a correlation coefficient of 0.9979 in the studied concentration range. The variation coefficients for repeatability and intermediate precision were less than 2 %. Accuracy reached a recovery rate of 100.20 % that is not statistically different from 100 %. It may be concluded that the validated method is specific, linear, precise and accurate and could be an advantageous alternative to the high resolution liquid chromatography method used at the lab setting.

REFERENCES

1. Núñez AJ, Vélez HT, Agüero J, González J, Naddeo F, De Simone F, et al. Isolation and quantitative analysis of phenolic antioxidants, free sugars, and polyols from mango (Mangifera indica L.) stem bark aqueous decoction used in Cuba as a nutritional supplement. J Agric Food Chem. 2002;50(4):762-6.

2. Garrido G, González D, Delporte C, Backhouse N, Quintero G, Núñez AJ, et al. Analgesic and anti-inflammatory effects of Mangifera indica L. extract (Vimang). Phytother Res 2001;15(1):18-21.

3. Sato T, Kawamoto A, Tamura A, Tatsumi Y, Fujii T. Mechanism of antioxidant action of pueraria glycoside (PG)-1 (an isoflavonoid) and mangiferin (a xanthonoid). Chem Pharm Bull (Tokyo). 1992;40(3):721-4.

4. Guha S, Ghosal S, Chattopadhyay U. Antitumor, immunomodulatory and anti- HIV effect of mangiferin, a naturally occurring glucosylxanthone. Chemotherapy. 1996;42(6):443-51.

5. Dai R, Gao J, Bi K. High-performance liquid chromatographic method for the determination and pharmacokinetic study of mangiferin in plasma of rats having taken the traditional Chinese medicinal preparation Zi-Shen pill. J Chromatogr Sci. 2004;42(2):88-90.

6. Dai R, Li K, Li Q, Bi K. Determination of mangiferin, jateorrhizine, palmatine, berberine, cinnamic acid, and cinnamaldehyde in the traditional Chinese medicinal preparation Zi-Shen pill by high-performance liquid chromatography. J Chromatogr Sci. 2004;42(4):207-10.

7. Nedialkov P, Kitanov G, Tencheva J. HPTLC densitomeric determination of justicidin B in Linum in vitro cultures. Acta Pharm. 1998;48:211-4.

8. Cao Y, Wang Y, Ye J. Differentiation of Swertia Mussotii Franch from Artemisiae Capillaris Herba by capillary electrophoresis with electrochemical detection. J Pharm Biomed Anal. 2005;39:60-5.

9. Nong C, He W, Fleming D, Pan L, Huang H. Capillary electrophoresis analysis of mangiferin extracted from Mangifera indica L. bark and Mangifera persiciformis C.Y. Wu et T.L. Ming leaves. J Chromatogr B. 2005;826:226-31.

10. Curiel H, Sordo L, Vélez H. Determinación cuantitativa por electroforesis capilar de zona de mangiferina en ingrediente farmacéutico activo VIMANG®. Revista CENIC Ciencias Químicas. 2007;38(2):337-43.

11. United States Pharmacopoeial Convention. USP XXX. United States Pharmacopoeia Convention. Validation of compendial methods. 30 ed. Rockville: Mack Printing; 2007.

12. Regulación No. 41. Validación de métodos analíticos. La Habana: CECMED; 2007.