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ISSN 1561-3011 (Electronic)

2011, Number 2

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Rev Cubana Invest Bioméd 2011; 30 (2)

Biomonitoring of workers exposed to pesticides

Lamadrid BAI, Romero AI, González MJE, Mandina CT

Language: Spanish
References: 20
Page: 235-244
PDF size: 277.96 Kb.


ABSTRACT

A biomonitoring of workers of the pesticide formulation plant from Managua municipality to know if the manipulation of pesticides described as potential chemical mutagens, had a genotoxic effect was carried out. The primary damage of DNA was studied by means of the comet assay and the chromosomal damage by chromosomal aberrations in lymphocytes and micronuclei in exfoliated cells of oral mucosa. For data analysis the DNA percentage in tail, the percentage of cells with chromosomal aberration and the percentage of cells with micronuclei were used. The frequency of chromosomal aberrations and of cells with micronuclei, both, in the exposed group and in the control one are within the interval reported for samples of Cuban population. The values of DNA percentage in tail, analyzed by comet assay in exposed group not showed significant differences before and after working day. The results analyzed demonstrate that manipulation of pesticides by this group of workers has not provoked any genetic damage perceptible by methods used.


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