Galán CE, Rodríguez JPA, Campos R, Ruiz L, Kouri JB, Guerrero JC, Ponce V, Chapa DJA

Language: Spanish
References: 0
Page: 20-25
PDF size: 591.13 Kb.

ABSTRACT

We compared the endothelial viability of rabbit corneas, preserved in three different storage medium for 48, 72 and 96 hours, being evaluated with trypan blue, light microscopy and transmission electron microscopy. Methods: The 20 cases were divided as follows: 8 corneas conserved in Optisol, 10 in MCB and 2 corneas in balanced saline solution, maintaining their temperature at 4o C. Results: We observed at 48 hours in balanced saline solution, endothelial cell destruction. Preserved corneas in MCB and Optisol, appreciated destruction in a 5%-10% of the cells at 72 hours of the preservation, after 96 hours in MCB the destruction is about 20%, and after 105 hours the destruction is 30% in the same medium. This cases, observed by the transmission electronic microscopy, confirm that the cells have been structurally preserved up to a 90 hours in Optisol and MCB. Conclusion: The medium produced in Mexico (MCB) preserved the corneal tissue for 90 hours, like Optisol, but being less expensive.