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Acta Ortopédica Mexicana

ISSN 2992-8036 (Electronic)
ISSN 2306-4102 (Print)
Órgano Oficial del Colegio Mexicano de Ortopedia y Traumatología
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2012, Number 3

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Acta Ortop Mex 2012; 26 (3)

Comparative study on animal model of acute Achilles tendon rupture with surgical treatment using platelet-rich plasma

Hernández-Martínez JC, Vásquez-CR, Ceja-CB, Fuentes-CCE, Sesma-JF, Benítez-AG
Full text How to cite this article

Language: Spanish
References: 8
Page: 170-173
PDF size: 34.53 Kb.


Key words:

calcaneous, tendon, rupture, platelet, plasma, animal model.

ABSTRACT

Objective: To compare the functional and histologicalal course of two animal model groups with acute Achilles tendon tears using platelet rich plasma. Material and methods: An open clinical trial was conducted with dogs donated by the animal facility of the Autonomous University of Puebla (BUAP, for its acronym in Spanish). Dogs were divided into 2 groups: a control group and a problem group. Intentional surgical Achilles tendon tear was performed to them. The Krackow technique was used to repair the tendon and the control group received platelet rich plasma (PRP) as a clot; the other group did not receive PRP. The dogs were seen at 4 weeks to check functionality using he Farell and Schwarz scale to assess the degree of limping. They were sacrificed at week 5; the tendons were removed and sent to the histopathology lab. Results: Functionality results according to the Farell and Schwarz scale showed grades I and II in the problem group, and grades IV and V in the control group. Histologically, the problem group showed moderate vascular proliferation and abundant fibroblastic proliferation. The control group had mild to moderate vascular proliferation and moderate fibroblastic proliferation. Conclusions: PRP improves tendon healing and this has repercussions on functional recovery.


REFERENCES

  1. Hernández I, Rossani G, et al: Plasma rico en plaquetas como inductor de reparación en la paniculopatía edematofibroesclerótica. Reingeniería de Tejidos. 2005; 7: 18-24.

  2. Maestro FA, Martínez RJ, Sánchez ZI, Rodríguez LL: Cirugía abierta y plasma rico en factores de crecimiento (PRGF) en roturas del tendón de Aquiles. Patología del aparato locomotor. 2007; 5: 79-82.

  3. Kawasumi M, Kitoh H, Siwicka KA, Ishiguro N: The effect of the platelet concentration in platelet-rich plasma gel on the regeneration of bone. J Bone Joint Surg 2008; 90-B: 966-73.

  4. Kaneps AJ, Parrot BG: Platelet-Rich Plasma: A new treatment for tendon and ligament injuries in horses. Parrot Equine Associates. Ohio State University College of Veterinary Medicine; 2007.

  5. Ferraz VCM, Ferrigno CRA, Schmaedecke A: Platelet concentration of platelet rich plasma from dogs, obtained through three centrifugation speeds. Braz J Vet Res Anim Sci Sao Paulo 2007; 44(6): 435-40.

  6. Mishra A, Pavelko T: Treatment of chronic elbow tendinosis with buffered platelet-rich plasma. Am J Sports Med 2006; 10: 1-6.

  7. Mazzucco L, et al: The use of autologous platelet gel to treat difficult-to-heal wounds: a pilot study. Transfusion 2004; 44: 1013-8.

  8. Maniscalco P, Gambera D, Lunati A, Vox G, Fossombroni V, Beretta R, Crainz E: The “Cascade” membrane: a new PRP device for tendon ruptures. Description and case report on rotador cuff tendon. Acta Biomed 2008; 79: 223-6.




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Acta Ortop Mex. 2012 May-Jun;26