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2011, Number 3

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Rev Cubana Plant Med 2011; 16 (3)

Antimicrobial action of Origanum vulgare L. essential oil against bacteria isolated from bovine milk

Bastos OME, Damé SLF, de Souza PL, Almeida SDB, Alves RMR, Braga de MJR

Language: Spanish
References: 19
Page: 260-266
PDF size: 93.28 Kb.


ABSTRACT

Introduction: bovine mastitis is a major problem in dairy production, mainly caused by Gram-positive bacteria. The search for active ingredients that act upon these microorganisms is growing due to occurrence of bacterial multiresistance.
Objectives: to assess the minimum bactericidal concentration (MBC) of Origanum vulgare L. (oregano) essential oil against bacteria isolated from mastitic milk.
Methods: the antimicrobial activity of O. vulgare essential oil was measured against 71 bacteria from the genera Streptococcus, Staphylococcus and Corynebacterium isolated from the bovine milk and against three pattern strains, that is, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The technique was dilution in microplate.
Results: the mean minimal bactericidal concentration (MBC) ranged from 0.23 % to 2 % against the bacteria isolated from bovine milk, with the lowest concentration for Streptococcus genus and the highest for coagulase-negative Staphylococcus. Regarding the pattern strains, the MBC was 3.17 % and 0.35 % for S. aureus and Escherichia coli respectively and showed no effect for Pseudomonas aeruginosa.
Conclusions: the results confirmed the in vitro activity of Origanum vulgare L. oil against the bovine mastitis-related bateria.


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