Oliveira SRN, Maximo SE, Ferraz PA, Muller AH, Bastos AC, Marins PM, Ferreira ME, Fâni DM

Language: Spanish
References: 11
Page: 135-139
PDF size: 113.33 Kb.

ABSTRACT

Introduction: Montrichardia linifera extracts showed high toxicity to Artemia salina, which indicated high biological potential for antitumoral, antibacterial, antifungal activities, and action against Trypanossoma cruzi.
Objective: to evaluate the antimalarial potential of the hexane extract from the Montrichardia linifera (Arruda) Schott leaves and to discover its phytochemical profile.
Methods: the phytochemical prospection was conducted by the precipitation method whereas the antiplasmodial activity was evaluated in vitro using the W2 Plasmodium falciparum clone.
Results: the phytochemical prospection just suggested the presence of steroids. Regarding the antiplasmodial activity after 24 hours at 100 µg/mL concentration, the parasite growth inhibition was 50.5 % but at other concentration ranges, significant inhibition was not observed. At 48 hours, the growth inhibition at 100 µg/mL concentrations was 34.7 %. After 72 hours, hemolysis was observed at 100 µg/mL and 50 µg/mL concentrations, so the percentage determination of the parasites was not possible.
Conclusions: the hexane extract obtained from the M. linifera leaves presented with low antimalarial potential and proved to be positive for steroids.

REFERENCES

1. World Health Organization. The World Health Report 2002: reducing risks, promoting healthy life. Geneva: WHO; 2002.

2. Judd WS, Campbell CS, Kellog EA, Stevens PF. Plant Systematics-a phylogenetic approach. Sunderland: Sinauer Associates; 2002. p. 576.

3. Silva LM, Oliveira MC, Prado AF, Silva RNO, Póvoa MM, Müller AH, et al. Anais XXI Congresso Brasileiro de Parasitologia e II Encontro de Parasitologia do Mercosul, 21. [CD-ROM/b]. Sociedade Brasileira de Parasitologia, Foz do Iguaçu; 2009

4. Prado AF, Silva LM, Oliveira MC, Silva RNO, Dolabela MF, Müller RCS, Müller AH, Amarante CB. Anais da Reunião Anual da Sociedade Brasileira de Química, 32. [CD-ROM/a]. Sociedade Brasileira de Química, Fortaleza; 2009

5. Dolabela MF. Triagem in vitro para atividade antitumoral e anti Trypanossoma cruzi de extratos vegetais, produtos naturais e susbstâncias sintéticas. Dissertação (Mestrado em farmacologia). Departamento de Fisiologia e Farmacologia, UFMG, Belo Horizonte; 1997. p. 130.

6. Matos FJA. Plantas Medicinais: guia de seleção e emprego de plantas usadas em fitoterapia no Nordeste do Brasil. 2° ed. Fortaleza: Imprensa Universitária; 2000. p. 344.

7. Trager W, Jensen JB. Human malaria parasites in contiuous culture. Science. 1976;193:673-5.

8. Rieckman KH, Sax LJ, Campbell GH, Mrena JF. Drug sensitivity of Plasmodium falciparum. An in vitro microtechnique. Lancet. 1978;6:22-3.

9. Carvalho LH. Quimioterapia experimental com extratos brutos de plantas quimicamente definidos (Mestrado). [Dissertação]. Belo Horizonte: Departamento de Parasitologia, Universidade Federal de Minas Gerais; 1990.

10. Cravo MP, Rosário VE. Aspectos de genética molecular da resistência aos fármacos antimaláricos. Boletim Biotecnologia. 2002;73:2-8.

11. Viana GMR, Machado RLD, Calvosa VSP, Póvoa MM. Mutations in the pfmdr1, cg2 and pfcrt genes in Plasmodium falciparum samples from endemic malaraia areas in Rondonia and Pará states, Brazilian Amazon region. Caderno Saúde Pública; 2006;22:2703-11.