Salgado CRM

Language: Spanish
References: 3
Page: 35-36
PDF size: 67.17 Kb.

ABSTRACT

The Herovici stain is a technique used to distinguish several tissue structures such as nucleus, cytoplasm and extracellular collagen, this technique is a picropolicromic stain. Sequentially application of several colorants like blue celestin, yellow metanil, fucshin acid and methyl blue, the tissues are stained red or blue in function of combination of methyl blue, picric acid and fucshin acid, according to the density of collagen in the tissue. Nuclear staining is black, cytoplasm is stained yellow-green, keratin, muscle and red blood cells in bright yellow and hyaline tissue in blue-green. This technique is little known but can be used for diagnosis, in tissue repair identify collagen type I and III proportions.

REFERENCES

1. Sarasquete C, Gutiérrez M. New tetrachromic VOF stain (type III-G.S) for normal and pathological fish tissues. Eur J Histochem 2005; 49 (2): 105-114.

2. Lamar M, Bancroft J, Gamble M. Connective tissues and stains. In: Bancroft J, Gamble M. Theory and practice of histological techniques. Churchill Livingstone Elsevier 2008: 146-149.

3. Herovici C. A polychrome stain for differentiating precollagen from collagen. Stain Technol 1963; 38: 204-205.