Arana-Trejo RM, Ignacio-Ibarra G, Baltazar-Arellano S, Tripp-Villanueva F, Flores-Peredo L, Báez-de la Fuente E, Rivas-Vera S, Garcés O, Solís-Anaya L, Duque J, Rodríguez-Castillo P

Language: Spanish
References: 14
Page: 68-72
PDF size: 120.38 Kb.

ABSTRACT

Background: Follicular Lymphoma (LF) is characterized by the presence of a t(14;18)(q32;q21) and that fusion of the genes Bcl-2/IgH; whose incidence presented variations geographical, ethnics and/or related to the technical of detections.
Objective: Evaluate frequency of presentation of Bcl-2/IgH by nested PCR and their correlation with karyotype in patients with LF; to be considered as a test to help of diagnoses.
Material and method: Retrospective study on 137 patients with LF were included, with median age 56 years (rank 24 to 80), 63 females and 74 males.
Results: Karyotype was made by conventional cytogenetics in bone marrow and/or peripheral blood, but only 71/137 samples were viable (52%) and the incidence observed of t(14;18) were low (6.6%), compared with literature (30-60%); so the sensitivity of karyotype (18%) is low, although with high specificity (100%). The study of Bcl-2/IgH was made in DNA amplified by nested PCR and was positive in 90/137 (66%) with breakpoints in the region MBR+ in 75/90 (83.3%) and in mcr+ 15 cases (16.6%).
Conclusion: The incidence of rearrangements Bcl-2/IgH it agrees with the reports of literature and the distribution of the breakpoints in Bcl-2 is high for mcr (16.6%) and it is resembled the reports of Argentina and USA. The sensitivity and specificity of the PCR for Bcl-2/IgH they were 80 and 86% respectively, which makes useful to the diagnosis and to be used during the evolution in patients with LF.

REFERENCES

1. Tan D, Homing S. Follicular Lymphoma: clinical features and treatment. Hematol Oncol Clin N Am 2008;22:863-882.

2. Ignacio G, Tripp F, Rodriguez P, Martínez MA, et al. Non Hodgkin lymphoma (NHL) distribution and clinical characteristic in a Mexican Registry with high frequency of T-cell NHL: a descriptive study on behalf of Mexican Hematology Study Group (MHSG). Blood (ASH Annual Meeting Abstracts) 2007;110:4407.

3. Harris L, Jaffe S, Stein H, et al. A Revised European-American classification of lymphoid neoplasms a proposal from the In ternational Lymphoma Study Group. Blood, 1994; 84(5):1361-1392.

4. Solal-Celigny P, Roy P, Colombat P, et al. Follicular lymphoma International Prognostic Index. Blood 2004;104:1258-1265.

5. Wilkins BS. Molecular genetics analysis in the assessment of lymphomas. Curr Diag Pathology 2004;10:351-359.

6. Johnson P, Swinbank K, MacLennan S, y col. Variability of polimerase chain reaction detection of the Bcl-2-IgH translocation in an international multicentre study. Ann Oncology 1999:1349-1354.

7. Aster JC, Longtine JA. Detection of Bcl2 rearrangement in follicular lymphoma. Am J Pathol 2002;160:759-763.

8. Noriega MF, DeBrasi C, Narbaitz M, Slavutsky I. Incidence of Bcl-2 gene rearrangements in Argentinean non-Hodgkin lymphoma patients: increased frequency of breakpoints outsider of MBR and MCR. Blood Cell Mol Diseases 2004;32:232-239.

9. Ismail SI, Sughayer MA, Al-Quadan TF, Qaqish BM, Tarawneh MS. Frequency of t(14;18) in follicular lymphoma patients: geographical or technical variation. Int J Lab Haemat 2009;31:535-543.

10. Igbal S, Jenner M, Summer K, y col. Reliable detection of clonal IgH/Bcl2 MBR rearrangement in follicular lymphoma: methodology and clinical significance. Br J Haematology 2004;124:325-328.

11. Johnson P. Polymerase Chain Reaction for detection of the t(14;18) translocation in lymphomas. In: Cotter FE. Methods in Molecular Medicine, Molecular Diagnosis of Cancer. Humana Press New Jersey: 1997; 63-74.

12. Radojkovic M, Ristic S, Colovic M, Cemericic MV, Krtolica K. Molecular characteristics and prognostic significance of Bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patientes. Neoplasma 2008; 55(5):421-427.

13. ISCN (2005), An International System for Human Cytogenetic Nomenclature, Mitelman F (ed). S. Karger, Basel, 2005.

14. www.ncbi.nlm.nih.gov/nuccore