2009, Number 4
Rev Mex Med Repro 2009; 1.2 (4)
Male germinal cells’ morphological changes related to age and oximetry in patients with fertility problems
Gómez AVE, Herrera ÁI, Uribe UMI, García PM, Villacorta AJA
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Language: Spanish
References: 20
Page: 145-150
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ABSTRACT
Objective: To determine the spermatic morphological variables in the seminal samples of two groups of patients of different ages.Material and methods: The samples of 101 couples were submitted to studies at the laboratory of the Rio de la Loza Perinatology and Obstetrics Hospital. Patients with two or more years of infertility were chosen, from January 2005 to August 2007. The cell samples obtained from this open population were processed by diagnostic methodology, and divided into two groups: one group (50 men) from 27 to 35 years and the other (51 men) from 36 to 45 years. The samples were obtained by masturbation with a minimum of a two-day abstinence. The morphological study was performed with aqueous stain of Wright Stain, with the modification of Bernard W. 1970 Dade Behring®. All seminal samples were processed in the gas analyzer ABL.330 Radiometer 1990, the results were compared with the findings. The preparation of the semen in the spermatic capacitation was done with the technique of isolation of two gradients, desconcentration Isolate upper-lower (Irving Scientific) in gradients of 40% and 90%, respectively. The cell counting process was done in the Makler chamber.
Results: In the first group the spermatic density found per patient was of 130.23n6 with 31.6% of normal germinal cells, head malformations added up to 27.7%, and tail malformations to 8%, with correlation coefficient of 0.45630. In the second group, spermatic density averages per ejaculation were 326n6, 52.2% were normal cells, with head malformations 33.1%, the coefficient was 0.4236; and tail malformations 20.2%, with coefficient of 0.0236. Blood gas analysis showed oxygen hypersaturation higher than the arterial levels, with a correlation coefficient of 0.0194 for normal samples.
Conclusions:The potentiometer results, with values between 7.2 and 7.6 pH OMS, do not show any significant relation due to age, adjust factor of 0.1. The base excess coefficient of -0.483 in the samples with theratogenesis excess. The early apoptotic events AnV and MM significantly were correlated with low motility fraction (r = 0.68 p = 0.05) in samples of twelve donors.
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