2011, Number 2
Intrauterine insemination with sex selection: a simple, cheap and effective modified technique of sperm capacitation
Neri VP, Gaona AR, Serviere ZC
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Language: Spanish
References: 9
Page: 77-81
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ABSTRACT
Objective: To know the results after applying the technique “modified” sperm capacitation with sex selection in couples with semen samples from normal semen parameters.Patients and method: Prospective study conducted from January 2010 to January 2011, which studied 22 couples who attended the Specialized Center Sterility and Human Reproduction, Hospital Angeles Mexico City, to undergo intrauterine insemination with sex selection. The couples were divided into two groups: A: couples with primary infertility who wanted to take advantage of intrauterine insemination for sex selection, and B: couples with secondary infertility where the cause of intrauterine insemination with sex selection was for social or cultural reasons. The two groups of patients underwent a protocol of ovulation induction with menotropins follicle stimulating hormone (FSH) and luteinizing hormone (LH) [Merapur®] to 150 IU per day, from day 3 of menstrual cycle and follicular monitoring; this dose was maintained until the development of three to five dominant follicles from 16 to 18 mm.
Results: 22 couples undergoing intrauterine insemination with sex selection were divided into two groups: A (n = 8) and B (n = 14), of the eight couples in group A, in five couples who selected the male sex there were two pregnancies (40%) of which two (100%) were male and in three couples who selected female sex a female pregnancy occurred (33.3%). Of the 14 couples of group B, in eight couples who selected male sex six pregnancies (75%) were obtained, of which five (83.3%) were males and one (16.6%) was a woman, and in six couples who selected female sex four pregnancies (66.6%) were obtainded, of which three (75%) were women and one (25%) was male. All pregnancies were unique because the stimulation protocol was used at low doses in order to ensure the not-recruitment of many follicles, but the growth of two or three follicles of high quality.
Conclusions: Our results are similar to those reported by Ericsson, who claims that his technique has an effectiveness of 78 to 85% when choosing males and from 73 to 75% when choosing female babies. Khatamee et al. (1999) and Check and Katsoff (1993) described that the swim-up technique is a very simple, economic and with statistically significant results for sex selection. And while the technique of this work is derived from the technique developed by Ericsson, we propose four miniswim make-up of 15 min instead of increasing concentrations of albumin in the culture medium, so, the results are similar to those obtained.
REFERENCES