Méndez-Rosito D, Rangel-López E, Aguirre-Cruz ML

Language: Spanish
References: 0
Page: 327-332
PDF size: 177.47 Kb.

ABSTRACT

Background: brain tumors are one of the leading cancers worldwide; in the National Institute of Neurology and Neurosurgery (INNN) these tumors are the leading cause of morbimortality.
Objective: Standardize biopsies, colletion, processing and storage biologic material of molecular studies.
Methods: with a previously signed surgical consent, a tumor and blood biopsy was done to 134 patients. Their DNA was extracted and a database was filled considering technical, ethical and legal aspects. In order to have optimal biologic material the procedure was standardized between the surgical and research laboratory teams.
Results: The biopsy, transportation, processing and storage were standardized. 134 patients were included (67 male and 67 female) with an average age of 46.28 years (range 15-81). The most frequently biopsied tumor was the meningioma (42%). The integrity of the obtained material was determined by agarose gel electrophoretic analysis.
Conclusion: the INNN biobank has a standardized system that biopsies, processes and stores optimum quality biologic material that will be the basis of future molecular studies.