Rivera-Tapia JA, Chávez-Bravo E, Lara-Sánchez N, Hernández-Aldana F, Rodríguez-Preval N

Language: Spanish
References: 10
Page: 202-206
PDF size: 59.09 Kb.

ABSTRACT

Mycoplasmas are a heterogeneous group of the smallest organisms capable of self replication, some mycoplasmas cause respiratory or urogenital diseases in humans. However, mycoplasmas often chronically colonize our respiratory and urogenital tracts without apparent clinical significance. In this respect, wall free mycoplasmas are among the few prokaryotes that can grow silently in close interaction with mammalian cells for a long period of time. However, prolonged interactions with mycoplasmas of seemingly low virulence could, through a gradual and progressive course, significantly affect many biologic properties of mammalian cells. DNases are important constituents of mycoplasmal membranes and may be involved in the acquisition of host nucleic acids required for growth. The objective was detected DNase activity in clinical Mycoplasma fermentans isolate. Digestion products were analyzed by loading a Tris-borato agarose gel, absorbance and medium for DNAse test. DNase activity was observed in strains tested and the levels of activity varied, Mycoplasma fermentans P140 showing greater activity. Result suggests DNases activities may be essential for growth and survival, this finding suggests that there is a mechanism of nucleotide acquisition.

REFERENCES

1. Rottem S. Interaction of mycoplasma with host cells. Physiol Rev 2003; 83: 417-432.

2. Sánchez HJA, Rivera TJA, Cortés MA, Tlecuitl MN, Méndez VF. Afecciones genitourinarias y micoplasmas. Rev Mex Patol Clin 2003; 50: 71-76.

3. Chowdhury IH, Munakata T, Koyanagi Y, Kobayashi S, Arai S, Yamamoto N. Mycoplasma can enhance HIV replication in vitro: A possible cofactor responsible for the progression of AIDS. Biochem Biophys Res Commun 1990; 170: 1365-1370.

4. Campo L, Larocque P, La Malfa T, Blackburn WD, Watson HL. Genotypic and phenotypic análisis of Mycoplasma fermentans straits isolated from different host tissues. J Clin Microbiol 1998; 36: 1371-1377.

5. Razin S. The mycoplasmas. Microbiol Rev 1978; 42: 414-470.

6. Gil C, Maldonado MG, Zenteno E, Yáñez A, Paz D, Cedillo L. Detección de micoplasmas en pacientes con asma. Rev Inst Nac Enf Resp Mex 1999; 12: 113-119.

7. Bendjennat M, Blanchard A, Loutfi M, Montagnier L, Bahraoui E. Role of Mycoplasma penetrans endonuclease P40 as a potential pathogenic determinant. Infect Immun 1999; 67: 4456-4462.

8. Minion FC, Coguen JD. Identification and preliminary characterization of a membrane-bound endonuclease in Mycoplasma pulmonis. Infect Immun 1986; 51: 352-354.

9. Roganti FS, Rosenthal AL. DNase of Acholeplasma spp. J Bacteriol 1983; 155: 802-805.

10. Paddenberg R, Weber A, Wulf S, Mannherz HG. Mycoplasma nucleases able to induce internucleosomal DNA degradation in cultured cells possess many characteristics of eukaryotic apoptotic nucleases. Cell Death Differ 1998; 5: 517-528.