2012, Number 2
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Investigación en Discapacidad 2012; 1 (2)
Effect of overexpression of angiogenin on cells isolated from human meniscus
Landa SC, Velasquillo MMC, García PH, Ibarra PLJC
Language: Spanish
References: 14
Page: 53-58
PDF size: 134.07 Kb.
ABSTRACT
The meniscus is characterized by low blood irrigation because 75% of the meniscus receives nutrients by passive diffusion through the synovial fluid, reason why the vasculature plays an essential role in early recovery of a damaged meniscus.
Methodology: In patients undergoing meniscectomy, were isolated and cultured fibrochondrocytes which were transfected with adenoviral vector Ad-GFP-Ang. The angiogenin level expression as well as the morphological changes, caused by the overexpression of the latter were evaluated by flow cytometry.
Results: We obtained 90% viability to transfected 4500 MOI of Ad-GFP-Ang, these cells increased the expression of GFP reporter gene which was considered a direct marker of protein expression of angiogenin, which induced phenotypic changes evidenced by expression of surface antigen CD34 (p ‹ 0.05), and morphological, given by the increase in size and complexity of the cell.
Conclusion: Over expression of angiogenin gene induces phenotypic and morphological changes that could be associated with early restoration of a meniscus injury.
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