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Antes Revista del Instituto Nacional de Enfermedades Respiratorias

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2012, Number 4

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Neumol Cir Torax 2012; 71 (4)

Evaluation of BacT ALERT 3D automatic system for mycobacteria isolates in National Reference Laboratory of tuberculosis, IPK

Martínez-Romero MR, Sardiña-Aragón M, García-León G, Mederos-Cuervo L, Vega-Riverón B, Díaz-Rodríguez R

Language: Spanish
References: 19
Page: 333-338
PDF size: 110.83 Kb.


ABSTRACT

Objective: To evaluate a BacT ALERT 3D automatic system for mycobacteria isolates. Methods: Were study 819 clinical samples received at the National Reference Laboratory of Tuberculosis, IPK, from August 2010 to November 2011. The samples were inoculated in parallel on Löwenstein Jensen medium and BacT ALERT 3D automatic system. The results obtained were analyzed and compared by number of isolates, time detection of growth (TDG), contamination rate (CR) and calculated the quality indicators of BacT ALERT 3D automatic system. Results: By Löwenstein Jensen (LJ) was obtained 99 (90%) positives isolates, 102 (92.7%) by BacT ALERT 3D and 89 (80.9%) by two methods simultaneously. El TDG average of BacT ALERT 3D was 16.67 days and 22.50 days for LJ. The CR was 7.4% and 6.7 % for LJ and BacT ALERT 3D, respectively. The concordance between BacT ALERT 3D and LJ was 97.82%. The sensitivity, specificity and Youden index obtained by BacT/ALERT 3D was 97.75%, 98.44% and 0.92, respectively. Conclusions: BacT/ALERT 3D system is a suitable method for recovering mycobacteria from clinical samples. It demonstrated a shorter TDG of mycobacteria of LJ medium. The LJ culture it must be used in combination with automatic systems for to assure a mycobacterial Mycobacterium recovery.


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