Bermúdez TD, Boffill CM, Valido DA, Martínez MCM, Iglesias RN

Language: Spanish
References: 13
Page: 43-48
PDF size: 120.96 Kb.

ABSTRACT

Photohemolysis test is used for determining the phototoxic potential of products that damage the erythrocyte membrane under the action of ultraviolet-visible light. Through this paper the photoirritant effect of Parthenium hysterophorus L., was evaluated as a risk assessment test, according to in vitro techniques in toxicology. Also secondary metabolites of the vegetable extract responsible for the effects we could perceive were determined. The results showed hemolysis at low concentrations and the hemolytic concentration 50 for irradiated and non-irradiated samples was 0,02 mg/ml and 0,05 mg/ml, respectively. On the other hand, the optical density values and the hemolytic factor obtained were lower than the classification range in each case. The presence of certain metabolites which could be, in part, and in different proportions responsible for the hemolytic effect that was obtained, was confirmed by phytochemical sifting. We got to a conclusion; the Parthenium hysterophorus L. soft extract is photoirritant.

REFERENCES

1. Vargas F, Rivas C, Zoltan T, Fuentes A, Padrón L, Díaz Y, et al. Photodegradation and in vitro phototoxicity of aceclofenac. Pharmazie. 2007;2(5):337-41.

2. Munday R, Smith BL, Munday CM. Structure-activity relationships in the haemolytic activity and nephrotoxicity of derivatives of 1,2- and 1,4-naphthoquinone. J Appl Toxicol. 2007;27(3):262-9.

3. Vargas F, Izzo C, Zoltan T, López V. Studies on the in vitro phototoxicity of the antidiabetes drug gliclazide. Avances en Química. 2006;1(3):3-12.

4. Wu TK, Wang YK, Chen YC, Feng JM, Liu YH, Wang TY. Identification of a Vibri furnissii oligopeptide permease and characterization of its in vitro hemolytic activity. J Bacteriol. 2007;189(22):8215-23.

5. González Madariaga Y, Boffill Cárdenas M, Bermúdez Toledo D, Castillo Alfonso O, Martínez Montalbán C, Martínez Bernal Y. Evaluación fotohemolítica in vitro de Cissus sicyoides L. y Achyranthes aspera L. Rev Cubana Plant Med. 2010;15(3):126-32.

6. Saucedo Hernández Y, Mohamad Safa B, González Bedia M, González San Miguel H, Bravo Sánchez L, Jorge Rodríguez E, et al. Especificidad de la cromatografía líquida de alta eficacia para evaluar estabilidad química basada en partenina del follaje seco pulverizado de Parthenium hysterophorus L. (escoba amarga). Rev Cubana Plant Med. 2010;15(1):3-17.

7. Saucedo Hernández Y, Mohamad Safa B, González Bedia M, González San Miguel H, Bravo Sánchez L, Jorge Rodríguez E, et al. Estabilidad del polvo de Parthenium hysterophorus L. (escoba amarga) basado en el contenido de partenina mediante cromatografía líquida de alta eficacia. Rev Cubana Plant Med. 2009;14(3):4-13.

8. Valdés FA. Actividad antimalárica in vitro y citotoxicidad de algunas plantas medicinales cubanas seleccionadas. Rev Inst Med Trop Sao Paulo. 2010;52(4):197-201.

9. González Madariaga Y, Boffill Cárdenas M, Bermúdez Toledo D, Sánchez Álvarez C, Castillo Alfonso O. Evaluación del ensayo de fotohemólisis en el sistema de Clasificación ABO Rh+. Medicentro Electrónica [Internet]. 2007 [citado 29 feb. 2011];11(1):[aprox. 5 p .]. Disponible en: http://www.vcl.sld.cu/sitios/medicentro/paginas%20de%20acceso/Sumario/ano%202007/v11n1a 07/evaluacion.htm

10. Miranda M, Cuéllar A. Manual de prácticas de laboratorio de Farmacognosia y productos naturales. La Habana: Pueblo y Educación; 2001.

11. Benavides T, Martinez V, Mitjans M, Infante MR, Moran C, Clapes P, et al. Assessment of the potential irritation and photoirritation of novel amino acid-based surfactants by in vitro methods as alternative to the animal tests. Toxicology. 2004;201(1-3):87-93.

12. Pignatello R, Noce C, Campisi A, Acquaviva R, Bucolo C, Puglisi G, et al. Evaluation of cell tolerability of a series of lipoamino acids using biological membranes and a biomembrane model. Curr Drug Deliv. 2007;4(2):109-21.

13. Águila Gil B, Meneses Castillo R, González Mata L, Madrigal Lesxay E, Fernández Fernández D. Extracto acuoso de Escoba amarga. Estudio preliminar de sus propiedades. Rev Cubana Plant Med. 2000;5(3):123-24.