2013, Number 2
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Rev Cubana Plant Med 2013; 18 (2)
Determination of anthocyanins by microwave assisted extraction in beans (Phaseolus vulgaris L.) commonly consumed in Antioquia, Colombia
Puertas-Mejía MA, Ríos-Yepes Y, Alberto RB
Language: Spanish
References: 15
Page: 288-297
PDF size: 142.82 Kb.
ABSTRACT
Introduction: apart from being an important nutritional source in Colombia, beans also provide a large amount of substances with potential beneficial effects on health, such as flavonoids and anthocyanins, among others, which stand out for their medicinal properties and potential positive action against some chronic diseases.
Objectives: compare the content of anthocyanins in the pericarp of the fruit of different varieties of beans with reference to the extraction method.
Methods: after dehydration and maceration, the bean fruit coat was subjected to solid-liquid and microwave assisted extraction with acidulated methanol. Anthocyanin content was determined by the pH differential method, and the cation radical α-α-diphenyl-ß-picrylhydrazyl (DPPH) method was used for determination of
in vitro antioxidant potential.
Results: microwave assisted extraction (MAE) proved to be more efficient than the conventional technique, with a considerable reduction in the amount of solvent, sample and extraction time. Anthocyanin content in the extracts obtained was between 8.33 and 25.5 mg cyanidin-3-glucoside/L. However, their antioxidant protection effect was similar and comparable to that of reference substances.
Conclusions: all extracts showed good protective capacity against free radicals. Microwave assisted extraction may be used as an alternative method for fast, efficient and effective evaluation of the content of bioactive substances in various matrices.
REFERENCES
Arias-Restrepo JH, Rengifo-Martínez T, Jaramillo-Carmona M. Manual Técnico: Buenas Prácticas Agrícolas en la Producción de Frijol Voluble. Corporación Colombiana de Investigación Agropecuaria-CORPOICA. Bogotá: CTP Print Ltda; 2007.
Geil P, Anderson J. Nutrition and health implications of dry beans: a review. J Am College Nutrition. 1994;13(6):549-58.
Mishra SB, Rao CV, Ojha SK. An analytical review of plants for anti diabetic activity with their phytoconstituent and mechanism of action: a review. International J Pharmacol Science Research. 2010;1(1):29-44.
Díaz AM, Caldas GV, Blair MW. Concentrations of condensed tannins and anthocyanins in common bean seed coats. Food Research International. 2010;43 (2):595-601.
Lin L-Z, Harnly JM, Pastor-Corrales MS. The polyphenolic profiles of common bean (Phaseolus vulgaris L.). Food Chemistry. 2008;107(1):399-410.
Buldini PL, Ricci L, Sharma JL. Recent applications of sample preparation techniques in food analysis. J Chromatography A. 2002;975(1):47-70.
Kataoka H. New trends in sample preparation for clinical and pharmaceutical analysis. TrAC. 2003;22(4):232-44.
Smith RM. Before the injection-modern methods of sample preparation for separation techniques. J Chromatography A. 2003;1000(1-2); 3-27.
Bose AK, Banik BK, Lavlinskaia N. More chemistry in a microwave. ChemTech. 1997;27(9):18-23.
LeBlanc G. Microwave-accelerated techniques for solid sample extraction. LC-GC. 1999;17(1):30-7.
Ganzler K, Salgó A, Valkó K. Microwave extraction. A novel sample preparation method for chromatography. J Chromatography A. 1986;371(1):299-306.
Lee J, Durst RW, Wrolstad RE. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study. J AOAC International. 2005;88(5):1269-78.
Puertas-Mejía MA, Zuleta-Montoya F, Rivera-Echeverry F. Capacidad antioxidante in vitro de comfrey (Symphytum officinale L.). Rev Cubana Plant Med. 2012;17(1):30-6.
Heimler D, Vignolini P, Dini M. Rapid tests to assess the antioxidant activity of Phaseolus vulgaris L. dry beans. J Agricultural Food Chemistry. 2005;53(8):3053-6.
Aparicio-Fernandez X, Yousef GG, Loarca-Pina G. Characterization of polyphenolics in the seed coat of Black Jamapa bean (Phaseolus vulgaris L.). J Agricultural Food Chemistry. 2005;53(11):4615-22.