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Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

2013, Number 1

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Rev Invest Clin 2013; 65 (1)

Operational mechanism modification of bone mechanostat in an animal model of nutritional stress: effect of propranolol

Pintos PM, Lezón CE, Bozzini C, Friedman SM, Boyer PM

Language: Spanish
References: 41
Page: 39-51
PDF size: 267.22 Kb.


ABSTRACT

Introduction. Propranolol (P) treatment exerts a preventive effect against the detrimental consequences to bone status in mildly chronically food-restricted growing rats (NGR) by an increment in cortical bone and by improving its spatial distribution. Objective. To study the effect of β-blocker on operational mechanism of bone mechanostat in an animal model of nutritional stress. Material and methods. Weanling male Wistar rats were randomly assigned to four groups: control (C), C + P (CP), NGR and NGR + P (NGRP). C and CP rats were fed freely with the standard diet. NGR and NGRP rats received, for 4 weeks, 80% of the amount of food consumed by C and CP respectively, the previous day, corrected by body weight. Propranolol (7 mg/kg/day) was injected ip 5 days per week, for four weeks in CP and NGRP rats. C and NGR received saline injections at an identical dosage regimen. Body weight and length were determined during the experimental period. Dietary intake was registered daily. Animals were sacrificed after 4 weeks of food restriction. Immediately, cuadriceps, femur and tibiae from each animal were dissected and weighed, and histomorphometric and mechanical studies were performed. Serum α-CTX, osteocalcin, intact PTH, calcium and phosphorous were determined. Body protein (% prot) was measured in all groups. Results. Food restriction induced detrimental effects on body and femoral growth, load-bearing capacity (Wf), % prot and cuadriceps weight in NGR vs. C (p ‹ 0.01). β-blocker did not modify anthropometric and bone morphometric parameters in NGRP and CP vs. NGR and C, respectively (p › 0.05). However, Wf NGRP vs. NGR was significantly higher (p ‹ 0.01). a-CTX was significantly higher in NGR vs. C (p ‹ 0.01). No significant differences were observed in α-CTX levels between CP, NGRP and C (p › 0.05). Serum osteocalcin, intact PTH, calcium and phospho- rous showed no significant difference between groups (p › 0.05). Conclusion. These results suggest that modeling increase in bone mass and strength in NGRP rats could be due to an anticatabolic interaction of the β -blocker propranolol on operational mechanism of bone mechanostat in an animal model of nutritional stress.


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