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Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2013, Number 2

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Rev Mex Patol Clin Med Lab 2013; 60 (2)

Comparison between automated count of SYSMEX XT800i and visual microscopy in the immature granulocyte flag

Pérez OJE, Vera DJ

Language: Spanish
References: 8
Page: 102-105
PDF size: 43.33 Kb.


ABSTRACT

Background: The automated analyzers are mechanism systems that are employed to realize the blood cellular count and leukocyte formula. In these days exist, some analyzers that have an extended differential count, in which includes immature granulocytes, reticulocytes count, and nucleated red blood cells. That’s why is important to analyze the precision on these new parameters. Objective: Analyze the performance of Sysmex XT1800i in the immature granulocyte parameter in comparison with the manual count. Material and methods: For an eight week period all the samples analyzed for complete blood count were recollected and only were selected the ones with immature granulocytes alarm. Comparison between automated and manual count was made. Manual count was done according some points of the National Committee for Clinical Laboratory Standards H20-A document. Results: 120 samples were analyzed, in which 20 did not demonstrated the presence of immature granulocytes by the manual count, in this samples Sysmex XT1800i was able to analyze 0.1x103, which is impossible in a manual differential. The correlation between both methods was r: 0.55. Conclusion: It is necessary the use of the manual count, for the confirmation of these parameter, because the presence of immature granulocytes in the blood film would make us think in an important infectious or inflammatory process.


REFERENCES

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  2. Weiland HK, Heihn H. Evaluation of the automated immature granulocyte count (IG) on Sysmex XE-2100 automated haematology analyser vs visual microscopy (NCCLS H20-A). Sysmex J Internat. 2002; 12: 63-70.

  3. Saenz F et al. Granulocitos inmaduros: Valores de referencia empleando analizador SYSMEX X-2100. Rev Mex Patol Clin. 2010; 57 (4): 163-169.

  4. Ike et al. Comparison of haematological parameters determined by the Sysmex KX - 2IN automated analyzer and the manual counts. BMC Clin Pathol. 2010; 10: 3.

  5. Briggs C et al. Performance evaluation of the Sysmex XE-2100, Automated Haematology Analyser. Sysmex J Internat. 1999; 9: 113-119.

  6. Balamurugan S, Jayapriya S, Jeya M, Ramesh R. Automated measurment of immature granulocytes: Performance characteristics and utility in routinne clinical practice. Pathol Res Internat. 2012; 2012: 6.

  7. National Committee for Clinical Laboratory Standards. Reference leukocyte differential count (Proportional) and evaluation of instrumental methods; Approved Standard, NCCLS document H20-A (ISBN 1-56238-131-8) NCCLS, 771 East Lancaster Avenue, Villanova, PA 19085, 1992.

  8. Sysmex Corporation Kobe (Japón). Manual de analizador hematológico automático XT-2000i -1800i instrucciones de uso, 2002.




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